CAJ | 학술논문

目的：观察生姜醇提物对四氯化碳所致大鼠肝纤维化进程中炎症相关因子的影响。方法将 SD雄性大鼠24只分为对照组（CON组，n＝8）、肝纤维化模型组（HF组，n＝8）及生姜醇提物干预组（ZGB组，n＝8）。HF组及 ZGB组大鼠背部皮下注射50％ CCL4橄榄油溶液3 mL/kg，每周2次，共8周，首次剂量加倍。同时 ZGB 组大鼠给予生姜醇提物溶液灌胃（300 mg/kg），CON组、HF组以等体积生理盐水灌胃，每天1次。8周后处死动物，采集血清，检测丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）水平。留取肝组织样本，测定羟脯氨酸（Hyp）水平；免疫印迹检测α-SMA蛋白的表达量；实时定量 PCR检测TNFα、IL-1及 IL-6的表达水平；HE染色观察肝组织的病理学改变。结果与 HF组比较，ZGB组血清 ALT、AST水平明显下降（P＜0．05）；肝组织 Hyp水平降低（P＜0．05）。免疫印迹结果显示，ZGB组α-SMA蛋白的表达量明显低于 HF组（P＜0．05）；实时定量PCR的结果显示，ZGB组肝组织TNFα、IL-1b及IL-6的表达水平较 HF组下降（P＜0．05）。光镜下ZGB组肝细胞变性和坏死程度较 HF组明显减轻。结论生姜醇提物具有较好的抗肝纤维化、保护肝细胞的作用，其机制之一可能是生姜醇提物能减少促炎因子TNFα、IL-1 b及 IL-6的释放，从而延缓或阻碍了肝纤维化进程。
목적：관찰생강순제물대사록화탄소치대서간섬유화진정중염증상관인자적영향。방법장 SD웅성대서24지분위대조조（CON조，n＝8）、간섬유화모형조（HF조，n＝8）급생강순제물간예조（ZGB조，n＝8）。HF조급 ZGB조대서배부피하주사50％ CCL4감람유용액3 mL/kg，매주2차，공8주，수차제량가배。동시 ZGB 조대서급여생강순제물용액관위（300 mg/kg），CON조、HF조이등체적생리염수관위，매천1차。8주후처사동물，채집혈청，검측병안산안기전이매（ALT）、천문동안산안기전이매（AST）수평。류취간조직양본，측정간포안산（Hyp）수평；면역인적검측α-SMA단백적표체량；실시정량 PCR검측TNFα、IL-1급 IL-6적표체수평；HE염색관찰간조직적병이학개변。결과여 HF조비교，ZGB조혈청 ALT、AST수평명현하강（P＜0．05）；간조직 Hyp수평강저（P＜0．05）。면역인적결과현시，ZGB조α-SMA단백적표체량명현저우 HF조（P＜0．05）；실시정량PCR적결과현시，ZGB조간조직TNFα、IL-1b급IL-6적표체수평교 HF조하강（P＜0．05）。광경하ZGB조간세포변성화배사정도교 HF조명현감경。결론생강순제물구유교호적항간섬유화、보호간세포적작용，기궤제지일가능시생강순제물능감소촉염인자TNFα、IL-1 b급 IL-6적석방，종이연완혹조애료간섬유화진정。
Objective To investigate effects of zingiber alcohol extract(ZGB)on the inflammation-associated cytokines in rats with carbon tetrachloride(CCL4)induced hepatic fibrosis.Methods 24 adult male SD rats were randomly divided into three groups:normal control group(CON,n=8),hepatic fibrosis model group(HF,n=8),and zingiber administration group(ZGB,n=8).The rats in the HF and ZGB groups were administrated by subcutaneous injection of 50%CCL4 3 mL/kg,twice per week for 8 weeks,the first time dosage was doubled.At the same time the ZGB group received daily intragastric ZGB(300 mg/kg a day),while the CON and HF groups received daily intragastric isovolumic normal saline for 8 weeks.The animals were executed at the end of 8 weeks.Serum samples were collected for detecting the content of ALT,AST.The liver samples were used for assessing the Hyp content,detectingα-SMA level by Western blotting and TNFα,IL-1 b,IL-6 mRNA levels by the real-time PCR.The histological ob-servation of the liver tissue was done with HE stain.Results As compared with the HF group,the serum levels of ALT and AST in the ZGB group were markedly decreased,and the content of Hyp in liver tissue was decreased(P<0.05).The Western blot re-sults showed that the expression amount ofα-SMA protein in the ZGB group was significantly lower than that in the HF group(P<0.05);the real-time quatitative PCR showed that the expression levels of TNFα,IL-1b,IL-6 were decreased compared with the HF group(P<0.05).The hepatocyte degeneration degrees observed by the optical microscope in the ZGB group were significantly alle-viated compared with the HF group.Conclusion ZGB has better anti-hepatic fibrosis and hepatocyte-protective effect,one of its mechanisms could be that ZGB could reduce the release of pro-inflammatory cytokines TNFα,IL-1b and IL-6,thus delay or hinder the progress course of liver fibrosis.