重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2014年
18期
2305-2307
,共3页
T淋巴细胞,调节性%肝炎,乙型,慢性%辅助性Th17细胞
T淋巴細胞,調節性%肝炎,乙型,慢性%輔助性Th17細胞
T림파세포,조절성%간염,을형,만성%보조성Th17세포
T-lymphocytes,regulatory%hepatitis-B,chronic%T-helper-1-7
目的:探讨小儿慢性乙型肝炎(CHB)患者不同疾病进展状态下Th17细胞/调节性 T细胞(Treg细胞)的平衡特点。方法入组16例健康对照(对照组)、60例小儿CHB患者(CHB组),采集外周血及血清,采用流式细胞仪检测 Fox3+Treg细胞及Th17细胞的频率,并用酶联免疫吸附试验方法(ELISA)检测血清中 Th17/Treg分化相关细胞因子。结果 CHB组的 Treg细胞、Th17细胞频率及Th17/Treg比值显著高于对照组,Th17频率与疾病进展呈正相关。CHB组患者血清中 IL-17A、IL-22及IL-23水平高于对照组(P<0.05)。结论小儿CHB组患者存在Th17/Treg的失衡。
目的:探討小兒慢性乙型肝炎(CHB)患者不同疾病進展狀態下Th17細胞/調節性 T細胞(Treg細胞)的平衡特點。方法入組16例健康對照(對照組)、60例小兒CHB患者(CHB組),採集外週血及血清,採用流式細胞儀檢測 Fox3+Treg細胞及Th17細胞的頻率,併用酶聯免疫吸附試驗方法(ELISA)檢測血清中 Th17/Treg分化相關細胞因子。結果 CHB組的 Treg細胞、Th17細胞頻率及Th17/Treg比值顯著高于對照組,Th17頻率與疾病進展呈正相關。CHB組患者血清中 IL-17A、IL-22及IL-23水平高于對照組(P<0.05)。結論小兒CHB組患者存在Th17/Treg的失衡。
목적:탐토소인만성을형간염(CHB)환자불동질병진전상태하Th17세포/조절성 T세포(Treg세포)적평형특점。방법입조16례건강대조(대조조)、60례소인CHB환자(CHB조),채집외주혈급혈청,채용류식세포의검측 Fox3+Treg세포급Th17세포적빈솔,병용매련면역흡부시험방법(ELISA)검측혈청중 Th17/Treg분화상관세포인자。결과 CHB조적 Treg세포、Th17세포빈솔급Th17/Treg비치현저고우대조조,Th17빈솔여질병진전정정상관。CHB조환자혈청중 IL-17A、IL-22급IL-23수평고우대조조(P<0.05)。결론소인CHB조환자존재Th17/Treg적실형。
Objective To study the frequency of peripheral blood T helper 17(Th17)and regulatory T cells(Treg)and their as-sociated cytokines level in child chronic hepatitis B(CHB)and to investigate the balance characteristics of Th1 7/Treg under differ-ent progression status in child CHB.Methods Peripheral blood and serum of 60 children with CHB and 16 healthy controls were collected for detecting the frequency of peripheral blood Fox3+Treg and Th1 7 by the flow cytometry and serum levels of Th1 7/Treg differentiation associated cytokine IL-17A,TGF-β,IL-22,IL-23,IL-6 and IL-1βwere detected by the enzyme-linked immu-nosorbent assay(ELISA).Results The frequency of peripheral blood Th1 7 and Treg,and the ratio of Th1 7/Treg in the CHB group were significantly higher than those in the healthy controls,the frequency of Th1 7 was positively correlated with disease progres-sion.In addition,serum IL-17A,IL-22 and IL-23 levels in the CHB children were higher than those in the healthy controls(P<0. 05).Conclusion Th17 and Treg play the important role in the mechanism of child CHB.The imbalance of Th17/Treg exists in child CHB.