CAJ | 학술논문

目的：探讨小檗碱对人肝癌细胞株 HEPG2蛋白表达的影响及其抗肿瘤作用的分子机制。方法采用蛋白质组学技术比较小檗碱处理人肝癌细胞株 HEPG2前、后蛋白表达的变化。结果筛选出差异表达蛋白共38个，16个蛋白表达上调，22个蛋白表达下调，质谱鉴定出10个蛋白点。结论小檗碱的抗肿瘤效应可能与过氧化物酶增殖物激活受体γ（PPARγ）信号转导通路有关，PPARγ可能是小檗碱的作用靶点。
목적：탐토소벽감대인간암세포주 HEPG2단백표체적영향급기항종류작용적분자궤제。방법채용단백질조학기술비교소벽감처리인간암세포주 HEPG2전、후단백표체적변화。결과사선출차이표체단백공38개，16개단백표체상조，22개단백표체하조，질보감정출10개단백점。결론소벽감적항종류효응가능여과양화물매증식물격활수체γ（PPARγ）신호전도통로유관，PPARγ가능시소벽감적작용파점。
Objective To research the effects of berberine on the protein expression of human hepatocellular carcinoma cell line HEPG2 for elucidating its antitumor mechanism.Methods The change of protein expression of human hepatocellular carcinoma cell line HEPG2 induced by berberine were analyzed by using comparative proteomics.Results A total of 38 differentially expressed protein spots were detected.16 protein spots were up-regulated and 22 protein spots were down-regulated.10 protein spots were successfully identified by the mass spectrum.Conclusion The berberine antitumor effect may be related with PPARγsignal trans-duction pathway,PPARγmay be the target of berberine.