CAJ | 학술논문

目的：采用高脂喂养与动脉内膜球囊损伤相结合的方法建立定位性兔髂动脉粥样硬化模型。方法：48只新西兰白兔随机分成两组：对照组：基础饲料+假手术（ n＝6）；模型组：饲以1%胆固醇、6%猪油的高脂饲料8周，进食高脂饲料后1周内皮剥脱（n＝42）。酶学法测定血脂全套。8周时进行髂动脉造影，处死对照组及部分模型组（n＝6）行光镜检查，部分行透射电镜及扫描电镜。结果：模型组中所有髂动脉都有不同程度的狭窄，大约15%~100%，平均61.47%；对照组未见明显狭窄。血清TG，TC，LDL-C在模型组中均显著增高（ P﹤0.01），而血清HDL-C在模型组均显著减少。组织病理学检查显示内皮细胞再生，形态不规则、较大，排列紊乱，以及明显内膜增厚，粥样斑块形成，其内主要含泡沫细胞、平滑肌细胞及纤维结缔组织增生。结论：高脂饮食及内皮损伤可在短期内成功复制定位性动脉粥样硬化模型，为动脉粥样硬化及再狭窄的研究提供实验基础。
목적：채용고지위양여동맥내막구낭손상상결합적방법건립정위성토가동맥죽양경화모형。방법：48지신서란백토수궤분성량조：대조조：기출사료+가수술（ n＝6）；모형조：사이1%담고순、6%저유적고지사료8주，진식고지사료후1주내피박탈（n＝42）。매학법측정혈지전투。8주시진행가동맥조영，처사대조조급부분모형조（n＝6）행광경검사，부분행투사전경급소묘전경。결과：모형조중소유가동맥도유불동정도적협착，대약15%~100%，평균61.47%；대조조미견명현협착。혈청TG，TC，LDL-C재모형조중균현저증고（ P﹤0.01），이혈청HDL-C재모형조균현저감소。조직병이학검사현시내피세포재생，형태불규칙、교대，배렬문란，이급명현내막증후，죽양반괴형성，기내주요함포말세포、평활기세포급섬유결체조직증생。결론：고지음식급내피손상가재단기내성공복제정위성동맥죽양경화모형，위동맥죽양경화급재협착적연구제공실험기출。
Objective To develop the focal atherosclerotic model induced by feeding high-fat diet combined with intimal balloon injury in rabbit iliac artery. Method New Zealand White rabbits( n=48 )were randomly divided into two groups:control group:a standard diet and sham operation(n=6);model group:an atherogenic diet with 1% cholesterol and 6% pig oil for 8 weeks and endothelial dedutation 1 week after atherogenic diet(n=42). Before and after 8 weeks of dietary intervention,blood samples were collected for enzymatic measure-ments of serum triglyceride( TG),total cholesterol( TC),low-density lipoprotein cholestrol( LDL-C)and high-density lipoprotein cholestrol( HDL-C)levels. At the end of the dietary intervention,iliac artery quantitative angiography was made and iliac arteries in con-trol and partial model group(n=6)were examined by light microscopy,some of which by scanning(SEM)and transmission electron mi-croscopy( TEM). Results All iliac arteries had various degrees of stenosis ranging from 15% to 100% with a average of 61. 47% in model group,but not in control group. Serum TG,TC and LDL-C were signficantly increased( P﹤0. 01)but serum HDL-C was remarkably de-creased in model group compared with control group,respectively. Histopathologic examination showed reendothelialization with large irregu-larly shaped and disoriented endothelial cells and marked intimal thicking with typical atherosclerotic plaque containing foam cells,smooth mucle cells and connective tissue elements. Conclusion Focal atherosclerotic model in rabbit iliac artery is successfully replicated by com-bining an atherogenic diet with endothelial dedutation within a short period of time,It builds up an experimental foundation in the researchs on atherosclerosis and restenosis.