农业科学研究
農業科學研究
농업과학연구
JOURNAL OF AGRICULTURAL SCIENCES
2014年
3期
34-38
,共5页
PCR-DGGE%16S rDNA%克隆技术%土壤细菌多样性%实验技术
PCR-DGGE%16S rDNA%剋隆技術%土壤細菌多樣性%實驗技術
PCR-DGGE%16S rDNA%극륭기술%토양세균다양성%실험기술
PCR-DGGE%16S rDNA%clone technique%soil bacterial diversity%experiment technology
PCR-DGGE是测定土壤微生物多样性常用的技术手段之一，但是提供的微生物多样性信息不够完整，如果与16S rDNA克隆技术相结合，微生物多样性信息更加丰富。 PCR-DGGE和16S rDNA克隆实验技术复杂，许多新手没有成熟可靠的经验可借鉴。以土壤细菌多样性研究方法为例，分别从 DNA 提取与纯化、PCR扩增、DGGE、胶片染色技巧以及16S rDNA 克隆技术等环节进行技术体系构建，可供利用该技术的研究人员参考。
PCR-DGGE是測定土壤微生物多樣性常用的技術手段之一，但是提供的微生物多樣性信息不夠完整，如果與16S rDNA剋隆技術相結閤，微生物多樣性信息更加豐富。 PCR-DGGE和16S rDNA剋隆實驗技術複雜，許多新手沒有成熟可靠的經驗可藉鑒。以土壤細菌多樣性研究方法為例，分彆從 DNA 提取與純化、PCR擴增、DGGE、膠片染色技巧以及16S rDNA 剋隆技術等環節進行技術體繫構建，可供利用該技術的研究人員參攷。
PCR-DGGE시측정토양미생물다양성상용적기술수단지일，단시제공적미생물다양성신식불구완정，여과여16S rDNA극륭기술상결합，미생물다양성신식경가봉부。 PCR-DGGE화16S rDNA극륭실험기술복잡，허다신수몰유성숙가고적경험가차감。이토양세균다양성연구방법위례，분별종 DNA 제취여순화、PCR확증、DGGE、효편염색기교이급16S rDNA 극륭기술등배절진행기술체계구건，가공이용해기술적연구인원삼고。
PCR-DGGE has been widely used for measuring soil microbial diversity, but microbial diversity information is limited for identification of microbial communities. If experiment method could completely combined with16S rDNA clone technique, its limitations could reduce, and conclusions also are accurate, reliable and with good repeatability. But the technology is technical process is more complex, and technical requirements is higher. In order to afford alutary experience or lessons to many new operators, experiment technology from the extraction of DNA, PCR amplification, DGGE experiment technology, dyeing and clone technique were described respectively.
