CAJ | 학술논문

目的：制备一种抗原封闭完全，蛋白成分保留完好，生物性能较好的鼠肾脱细胞基质支架，并对其体内生物相容性进行分析。方法：取健康SD大鼠，采用腹主动脉在体灌注去污剂的方法制备全肾脱细胞基质支架，对处理后的支架材料进行形态结构观察，主要成分分析；将其支架进行皮下包埋检测体内细胞相容性。结果：经脱细胞处理后，肾支架形态上具有三维立体空间结构，肉眼可见其内血管脉络；DNA残留量不及正常组3%；HE、透射电镜（TEM）、PAS等观察细胞成分已完全去除，细胞外基质网络结构保留完整；免疫荧光显示细胞外基质成分Ⅳ型胶原（collagen Ⅳ）、层黏连蛋白（laminin）及纤维黏连蛋白（fibronectin）均呈强阳性表达，未见明显细胞核成分残留；皮下植入结果显示，初期炎症反应明显，随着时间推移，炎症减轻，支架内逐渐有血管长入，基质逐渐降解。结论：经去污剂灌注处理的脱细胞肾基质，可有效清除肾内所有细胞成分，较好地保留细胞外基质支架且形态完好，具有良好的组织相容性。
목적：제비일충항원봉폐완전，단백성분보류완호，생물성능교호적서신탈세포기질지가，병대기체내생물상용성진행분석。방법：취건강SD대서，채용복주동맥재체관주거오제적방법제비전신탈세포기질지가，대처리후적지가재료진행형태결구관찰，주요성분분석；장기지가진행피하포매검측체내세포상용성。결과：경탈세포처리후，신지가형태상구유삼유입체공간결구，육안가견기내혈관맥락；DNA잔류량불급정상조3%；HE、투사전경（TEM）、PAS등관찰세포성분이완전거제，세포외기질망락결구보류완정；면역형광현시세포외기질성분Ⅳ형효원（collagen Ⅳ）、층점련단백（laminin）급섬유점련단백（fibronectin）균정강양성표체，미견명현세포핵성분잔류；피하식입결과현시，초기염증반응명현，수착시간추이，염증감경，지가내축점유혈관장입，기질축점강해。결론：경거오제관주처리적탈세포신기질，가유효청제신내소유세포성분，교호지보류세포외기질지가차형태완호，구유량호적조직상용성。
Objective:To prepare the kidney decellularized matrix bio-derived scaffold with lower antige-nicity, complete protein components and good biological properties in rats and evaluate its biocompatibility in vivo. MethodBy the perfusion with chemical detergents through the abdominal aorta, the acellular scaffolds of kidney were prepared in SD rats. Then the decellularized scaffolds and native kidneys were analyzed through ge-nomic DNA content analysis, transmission electron microscopy (TEM), HE, PAS, Massons and immunolfuores-cence. Acellular scaffolds were transplanted in situ to evaluate the biocompatibility. ResultAfter being treated by a variety of reagents, cells within kidney tissue had been largely removed. The acellular scaffold had forms of three-dimensional network structure with integrated vascular network. DNA contents of the acellular renal ECM in the experimental group were 3%, less than that in the normal group. Immunohistochemistry showed a lot of collagen ifbers and no signiifcant residual cell debris within scaffold was found. The gross and histological obser-vation of subdermal implantation showed the inlfammatory reactions were severe onset. There was an increased collagen ifber density and angiogenesis, and the transplanted acellular matrix was gradually degraded and ab-sorbed. Conclusion:The renal scaffolds prepared in rats are acellular but have the structure of three-dimensional network. The reticular structure of the extracellular matrix is well preserved, which has good biocompatibility in vivo as tissue engineering scaffold for renal reconstruction and regeneration.