武警医学
武警醫學
무경의학
MEDICAL JOURNAL OF THE CHINESE PEOPLE'S ARMED POLICE FORCES
2014年
9期
912-915
,共4页
CML28%WT1%急性白血病%相关性
CML28%WT1%急性白血病%相關性
CML28%WT1%급성백혈병%상관성
CML28%WT1%acute leukemia%correlation
目的：探讨CML28与WT1分子在急性白血病（ acute leukemia ， AL）患者原代骨髓细胞中的表达及表达相关性。方法收集华中科技大学附属同济医院2009-01至2009-06收住入院的29例初治AL患者骨髓单个核细胞，采用Trizol一步法提取细胞总RNA，逆转录反应合成cDNA，通过反转录PCR （reverse transcription PCR，RT-PCR）及实时定量PCR（Realtime PCR）检测CML28与WT1分子的mRNA表达情况，并通过相对定量公式2-ΔΔCT计算CML28及WT1分子的mRNA相对表达水平。结果29例初治AL患者骨髓单个核细胞同时高表达CML28及WT1分子，二者表达水平呈正相关关系（ r＝0．462，P＝0．008）。结论 CML28及WT1分子同时高表达于各种急性白血病，且呈明显的正相关关系。
目的：探討CML28與WT1分子在急性白血病（ acute leukemia ， AL）患者原代骨髓細胞中的錶達及錶達相關性。方法收集華中科技大學附屬同濟醫院2009-01至2009-06收住入院的29例初治AL患者骨髓單箇覈細胞，採用Trizol一步法提取細胞總RNA，逆轉錄反應閤成cDNA，通過反轉錄PCR （reverse transcription PCR，RT-PCR）及實時定量PCR（Realtime PCR）檢測CML28與WT1分子的mRNA錶達情況，併通過相對定量公式2-ΔΔCT計算CML28及WT1分子的mRNA相對錶達水平。結果29例初治AL患者骨髓單箇覈細胞同時高錶達CML28及WT1分子，二者錶達水平呈正相關關繫（ r＝0．462，P＝0．008）。結論 CML28及WT1分子同時高錶達于各種急性白血病，且呈明顯的正相關關繫。
목적：탐토CML28여WT1분자재급성백혈병（ acute leukemia ， AL）환자원대골수세포중적표체급표체상관성。방법수집화중과기대학부속동제의원2009-01지2009-06수주입원적29례초치AL환자골수단개핵세포，채용Trizol일보법제취세포총RNA，역전록반응합성cDNA，통과반전록PCR （reverse transcription PCR，RT-PCR）급실시정량PCR（Realtime PCR）검측CML28여WT1분자적mRNA표체정황，병통과상대정량공식2-ΔΔCT계산CML28급WT1분자적mRNA상대표체수평。결과29례초치AL환자골수단개핵세포동시고표체CML28급WT1분자，이자표체수평정정상관관계（ r＝0．462，P＝0．008）。결론 CML28급WT1분자동시고표체우각충급성백혈병，차정명현적정상관관계。
Objective To study the expression level and correlation between CML 28 and WT1 in acute leukemia ( AL) pa-tients’ bone marrow( BM) primary cells.Methods Bone marrow mononuclear cells ( MMNC) were collected from 29 AL patients who were initially treated and admitted to the Tongji Hospital affiliated to Huazhong University of Science&Technology from January to June in 2009.Total mRNA was extracted from MMNC of AL patients using Trizol reagents , and the first cDNA was synthesized using reverse transcription kits .Their mRNA expression levels were detected in AL patients by RT-PCR and real time PCR and their mRNA expression levels were quantitated by relevant quantitative formula 2 -ΔΔCT .Then the correlation coefficient was calculated using SPSS 17.0 statistical software.Results There was a high expression level of CML28 and WT1 in the MMNC of AL patients at the same time, with significant difference between their mRNA expression leuels (r=0.462,P=0.008).Conclusions CML28 and WT1 are highly expressed in a variety of acute leukemia , and they have an obviously positive correlation .
