CAJ | 학술논문

目的：探讨人钠耦合中性氨基酸转运蛋白1（ SNAT1）和上皮膜蛋白1（ EMP1）在胃癌组织及胃癌细胞株中的表达水平，分析两者在胃癌组织中表达与临床病理特征的关系。方法采用免疫组化SP法检测82例胃癌组织和67例对应癌旁组织样本中SNAT1和EMP1的蛋白表达情况，根据二级计分法分为低表达（＜5分）和高表达（≥5分），分析两者不同表达水平与胃癌临床病理特征的关系。同时采用Western blotting检测20例胃癌组织及其癌旁组织、正常胃黏膜上皮细胞株GES1和胃癌不同分化细胞株（未分化BGC823、低分化AGS、中分化SGC7901和高分化MKN28）中SNAT1和EMP1的蛋白水平。结果胃癌组织的 SNAT1高表达率高于癌旁组织（67?1％ vs．34?3％， P＜0?05），而 EMP1高表达率则低于癌旁组织（40?2％ vs．62?7％， P＜0?05）。胃癌组织中SNAT1与EMP1表达呈负相关（r＝0?272， P＝0?014），且两者表达与临床分期和分化程度均有关；另外，SNAT1与年龄、淋巴结转移有关，EMP1与浸润深度有关。胃癌细胞株中的SNAT1蛋白水平均高于癌旁组织和CES1细胞，而EMP1蛋白水平则相反，差异均有统计学意义（ P＜0?05）。结论 SNAT1表达在胃癌组织及细胞株中分别较癌旁组织和正常胃黏膜细胞株高，EMP1则相反，胃癌组织中两种蛋白均与临床分期和分化程度有关，提示两者可能在胃癌的发生发展中起重要作用，可用于辅助胃癌的诊断和病情评估。
목적：탐토인납우합중성안기산전운단백1（ SNAT1）화상피막단백1（ EMP1）재위암조직급위암세포주중적표체수평，분석량자재위암조직중표체여림상병리특정적관계。방법채용면역조화SP법검측82례위암조직화67례대응암방조직양본중SNAT1화EMP1적단백표체정황，근거이급계분법분위저표체（＜5분）화고표체（≥5분），분석량자불동표체수평여위암림상병리특정적관계。동시채용Western blotting검측20례위암조직급기암방조직、정상위점막상피세포주GES1화위암불동분화세포주（미분화BGC823、저분화AGS、중분화SGC7901화고분화MKN28）중SNAT1화EMP1적단백수평。결과위암조직적 SNAT1고표체솔고우암방조직（67?1％ vs．34?3％， P＜0?05），이 EMP1고표체솔칙저우암방조직（40?2％ vs．62?7％， P＜0?05）。위암조직중SNAT1여EMP1표체정부상관（r＝0?272， P＝0?014），차량자표체여림상분기화분화정도균유관；령외，SNAT1여년령、림파결전이유관，EMP1여침윤심도유관。위암세포주중적SNAT1단백수평균고우암방조직화CES1세포，이EMP1단백수평칙상반，차이균유통계학의의（ P＜0?05）。결론 SNAT1표체재위암조직급세포주중분별교암방조직화정상위점막세포주고，EMP1칙상반，위암조직중량충단백균여림상분기화분화정도유관，제시량자가능재위암적발생발전중기중요작용，가용우보조위암적진단화병정평고。
Objective To explore the expressions of sodium coupled neutral amino acid transporter 1 ( SNAT1) and epithelial membrane protein ( EMP1) in gastric cancer tissue and cell lines, and analyze their relationship with clinicopathological features of gastric cancer. Methods The immunohistochemistry SP method was used to detect the expressions of SNAT1 and EMP1 in tissue sec-tions from 82 gastric cancer and 67 paracancerous tissues from our hospital. The expression level was divided into low-level expression (<5 points) and high-level expression(≥5 points) according to the two grade score. Then the relationships between expressions of SNAT1 and EMP1 and clinicopathological variables were analyzed. Meanwhile, the Western blotting method was employed to detect the protein level of SNAT1 and EMP1 in 20 paired cancer and paracancerous tissues as well as normal gastric mucosa cell ( GES-1) and different cancer cell lines of various differentiation( BGC823, SGC7901, AGS and MKN28) . Results The high-level expression rate of SNAT1 in cancer tissues were 67?1%, higher than 34?3% in paracancerous tissues ( P<0?05) , while the high-level expression rate of EMP1 was 40?2% in cancer tissues, lower than 62?7% in paracancerous tissues ( P<0?05) . The expression of SNAT1 was negative-ly correlated with EMP1 in cancer tissues ( r=-0?272, P=0?014) . Both expressions were related with clinical stage and differentiation degree. In addition, the SNAT1 expression was related with age and lymph node metastasis, and the EMP1 expression was related with invasive depth ( P<0?05) . Compared with the paracancerous tissues and normal gastric mucosa cell, there existed higher protein level of SNAT1 but lower protein level of EMP1 in gastric cancer tissue and cell lines (P<0?05). Conclusion Both SNAT1 and EMP1 were related with clinical stage and differentiation degree, suggesting playing a important role in the development of gastric cancer. Both proteins can facilitate the diagnosis and evaluation of gastric cancer.