CAJ | 학술논문

目的：观察新型胆道双极射频消融导管HabibTM EndoHPB设置不同功率及持续时间的消融效果。方法在恒温下（37?0℃）使用新型胆道射频消融导管对新鲜离体猪肝进行实验，分别设置功率5、10和15W，时间60、90、120和150s。观察在不同功率/时间参数时，消融区域的温度、宏观（短轴/长轴）及微观的变化。结果设置功率5W、时间60～150s，消融区域的最高温度为42?0～54?0℃；10W、60～150s，最高温度为52?0～70?0℃；15W、60～120s，最高温度为62?0～77?0℃。10W和60、90、120及150s时，短轴长度（mm）分别为8?5±0?8、9?0±0?8、9?5±0?8及10?0±0?8；长轴长度（mm）分别为21?0±1?0、23?5±1?0、25?5±1?0及30?0±1?0。5、10、15W时，短轴和长轴长度均有小幅增大，其中功率为10W及15W时，两组差异无统计学意义（ P＞0?05）。镜下消融区域均出现肝组织解离、细胞间距变大、细胞广泛变性，程度随着功率和时间增加而加重，且在10W、150s及15W、120s时，出现片状液化性坏死。结论 HabibTM EndoHPB导管设置功率10W、时间90至120s进行消融比较合适，在临床实际操作中可参考本实验结果进行适当的调整。
목적：관찰신형담도쌍겁사빈소융도관HabibTM EndoHPB설치불동공솔급지속시간적소융효과。방법재항온하（37?0℃）사용신형담도사빈소융도관대신선리체저간진행실험，분별설치공솔5、10화15W，시간60、90、120화150s。관찰재불동공솔/시간삼수시，소융구역적온도、굉관（단축/장축）급미관적변화。결과설치공솔5W、시간60～150s，소융구역적최고온도위42?0～54?0℃；10W、60～150s，최고온도위52?0～70?0℃；15W、60～120s，최고온도위62?0～77?0℃。10W화60、90、120급150s시，단축장도（mm）분별위8?5±0?8、9?0±0?8、9?5±0?8급10?0±0?8；장축장도（mm）분별위21?0±1?0、23?5±1?0、25?5±1?0급30?0±1?0。5、10、15W시，단축화장축장도균유소폭증대，기중공솔위10W급15W시，량조차이무통계학의의（ P＞0?05）。경하소융구역균출현간조직해리、세포간거변대、세포엄범변성，정도수착공솔화시간증가이가중，차재10W、150s급15W、120s시，출현편상액화성배사。결론 HabibTM EndoHPB도관설치공솔10W、시간90지120s진행소융비교합괄，재림상실제조작중가삼고본실험결과진행괄당적조정。
Objective To estimate the effects of a novel radiofrequency ( RF) ablation catheter, the HabibTM EndoHPB, with various settings of powers and times. Methods We used resected fresh pig livers to examine at 37?0℃. The RF application was per-formed step by step at 5, 10, and 15 W power and 60, 90, 120,150s, respectively. The change of temperature, macroscopic( the lengths of the short and long axes) and microscopic findings of the ablation area were evaluated at each setting. Results The highest temperature of the ablation area at 5W power and 60, 90, 120 and 150s were 42?0℃ to 54?0℃, at 10W power and 60, 90, 120 and 150s were 52?0℃ to 70?0℃, and at 15W power and 60, 90 and 120s were 62?0℃ to 77?0℃. The lengths of the short axis of the ab-lation area at 10W power and 60, 90, 120 and 150s were (8?5±0?8)mm, (9?0±0?8)mm, (9?5±0?8)mm and (10?0±0?8)mm, re-spectively. The lengths of the long axis at 10W power and 60, 90,120 and 150s were (21?0±1?0)mm, (23?5±1?0)mm, (25?5± 1?0)mm and (30?0±1?0)mm, respectively. Although the lengths of the short and long axes at 5, 10 and 15W increased gradually with power, there were no obvious differences in either short or long axis lengths between 10 and 15W power( P<0?05) . Microscopic examination of the ablationareashowed liver tissue dissociationed, cell spacing increased and extensive cellular degenerationed. The de-gree increased as power/time increased. And liquefactive necrosis sheet was appeared at 10W power and 120s or 15W power and 150s. Conclusion Although the clinical ablation setting at 10W power and 90 to 120s is suitable, ultimately the ablation power and time should be adjusted according to our experimental result.