海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
9期
141-143
,共3页
陆春燕%龙伟%温露%刘素娜%陈钢
陸春燕%龍偉%溫露%劉素娜%陳鋼
륙춘연%룡위%온로%류소나%진강
牛血清白蛋白%乳酸-羟基乙酸共聚物%包封率%聚乙烯醇%体外表征
牛血清白蛋白%乳痠-羥基乙痠共聚物%包封率%聚乙烯醇%體外錶徵
우혈청백단백%유산-간기을산공취물%포봉솔%취을희순%체외표정
Bovine serum albumin%Poly ( lactic-co-glycolic acid )%Encapsulation efficiency%Polyvinyl alcohol ( PVA )%In vitro characteristics
目的:以乳酸-羟基乙酸共聚物(PLGA)为载体,制备载牛血清白蛋白(BSA)PLGA微球,测定包封率,并表征其体外性质。方法采用复乳-溶剂挥发法制备载BSA PLGA微球,联合应用硫酸钠沉淀聚乙烯醇(PVA)和二喹啉甲酸(BCA)法测定其包封率,光学显微镜观察形态,激光粒度仪测定粒径大小及分布,并考察其体外释药性能。结果制得微球形态圆整、无粘连,微球平均粒径为103μm,包封率为(98.5±0.68)%。体外释放试验表明有较好的缓释性能,且符合Ritger-Peppas模型。结论制备得到包封率高,有明显缓释效果的BSA PLGA微球,且硫酸钠的使用可显著消除乳化剂PVA对包封率测定的干扰。
目的:以乳痠-羥基乙痠共聚物(PLGA)為載體,製備載牛血清白蛋白(BSA)PLGA微毬,測定包封率,併錶徵其體外性質。方法採用複乳-溶劑揮髮法製備載BSA PLGA微毬,聯閤應用硫痠鈉沉澱聚乙烯醇(PVA)和二喹啉甲痠(BCA)法測定其包封率,光學顯微鏡觀察形態,激光粒度儀測定粒徑大小及分佈,併攷察其體外釋藥性能。結果製得微毬形態圓整、無粘連,微毬平均粒徑為103μm,包封率為(98.5±0.68)%。體外釋放試驗錶明有較好的緩釋性能,且符閤Ritger-Peppas模型。結論製備得到包封率高,有明顯緩釋效果的BSA PLGA微毬,且硫痠鈉的使用可顯著消除乳化劑PVA對包封率測定的榦擾。
목적:이유산-간기을산공취물(PLGA)위재체,제비재우혈청백단백(BSA)PLGA미구,측정포봉솔,병표정기체외성질。방법채용복유-용제휘발법제비재BSA PLGA미구,연합응용류산납침정취을희순(PVA)화이규람갑산(BCA)법측정기포봉솔,광학현미경관찰형태,격광립도의측정립경대소급분포,병고찰기체외석약성능。결과제득미구형태원정、무점련,미구평균립경위103μm,포봉솔위(98.5±0.68)%。체외석방시험표명유교호적완석성능,차부합Ritger-Peppas모형。결론제비득도포봉솔고,유명현완석효과적BSA PLGA미구,차류산납적사용가현저소제유화제PVA대포봉솔측정적간우。
OBJECTIVE To prepare poly (lactic-co-glycolic acid)(PLGA) microspheres loaded with bovine serum albumin ( BSA ) and study their characterization.METHODS BSA PLGA microspheres were prepared by double emulsion-solvent evaporation method.The encapsulation efficiency of BSA in the microspheres was determined by BCA assay after precipitating PVA with sodium sulfate.Light microscope was used for the observation of micro-spheres.Mean diameter and particle size distribution were determined by laser scattering technique ,and the release of BSA from microspheres was also performed.RESULTS Light microscope showed the microspheres were spheric morphology and the particle size was homogeneous.Meandiameter and encapsulation efficiency of microspheres were 103μm and (98.5 ±0.68 )%respectively.The in vitro test showed an obvious sustained release activity and the re-lease kinetics in vitro obeyed Ritger-Peppas equation.CONCLUSION The microspheres were obtained with high encapsulation efficiency and obvious sustained release effect.The addition of sodium sulfate can significant reduce the measurement interference that come from PVA.