CAJ | 학술논문

目的：建立高效液相色谱法测定腐竹中甲醛次硫酸氢钠的含量。方法样品中甲醛次硫酸氢钠分解释放的甲醛与2，4-二硝基苯肼反应生成的衍生物经HPLC检测；色谱柱：Agilent TC-C18（250mm ×4.6mm，5μm），流动相：乙腈-水（70∶30）；流速1.0mL· min 1；检测波长355nm；柱温30℃。结果甲醛浓度在0～40μg 范围内与其衍生物峰面积线性关系良好（ r＝0.9997），平均回收率为98.2％，方法最低检出浓度为0.62μg· g1。结论本法简单、准确，可用于腐竹中甲醛次硫酸氢钠的检测。
목적：건립고효액상색보법측정부죽중갑철차류산경납적함량。방법양품중갑철차류산경납분해석방적갑철여2，4-이초기분정반응생성적연생물경HPLC검측；색보주：Agilent TC-C18（250mm ×4.6mm，5μm），류동상：을정-수（70∶30）；류속1.0mL· min 1；검측파장355nm；주온30℃。결과갑철농도재0～40μg 범위내여기연생물봉면적선성관계량호（ r＝0.9997），평균회수솔위98.2％，방법최저검출농도위0.62μg· g1。결론본법간단、준학，가용우부죽중갑철차류산경납적검측。
OBJECTIVE To establish a HPLC method for quantitation of sodium formaldehyde sulfoxylate in dried beancurd sticks.METHODS The formaldehyde released from the decomposition of sodium bisulfoxylate form-aldehyde in the sample reacted with 2,4-dinitrophenylhydrazine (DNPH) and was determined by HPLC.Agilent TC-C18 column (250mm ×4.6mm,5μm) was used with temperature at 30℃.The mobile phase consisted of acetonitril-water(70∶30)and the flow rate was 1.0mL· min -1,The detective wavelength was 355nm.RESULTS The calibra-tion curve of formaldehyde was linear within the range of 0 ~40μg ( r =0.999 7 ) , the average recovery rate was 98.2%.The minimum detectable limit was 0.62μg · g -1.CONCLUSION The method is simple and accurate , which can be used for the detecting of sodium for maldehyde sulfoxylate in dried beancurd sticks .