CAJ | 학술논문

目的：观察松郁安神方对睡眠剥夺大鼠血清细胞因子的影响，进一步探讨松郁安神方调节睡眠的机制。方法采用“改良多平台睡眠剥夺法”构建睡眠剥夺大鼠模型，以正常笼养及大平台作为对照组，采用酶联免疫吸附法测定睡眠剥夺大鼠经松郁安神方治疗后，血清细胞因子IL-1β、TNF-α水平的变化。结果松郁安神方组血清TNF-α高于REM睡眠剥夺组（P＜0.05）；松郁安神方组血清IL-1β较正常对照组、大平台环境对照组及REM睡眠剥夺组无显著性差异（P＞0.05）。结论松郁安神方可能通过调节血清细胞因子TNF-α的含量从而调节睡眠，且通过对睡眠剥夺大鼠睡眠的调节继而改善睡眠剥夺所致机体的过度能量代谢。
목적：관찰송욱안신방대수면박탈대서혈청세포인자적영향，진일보탐토송욱안신방조절수면적궤제。방법채용“개량다평태수면박탈법”구건수면박탈대서모형，이정상롱양급대평태작위대조조，채용매련면역흡부법측정수면박탈대서경송욱안신방치료후，혈청세포인자IL-1β、TNF-α수평적변화。결과송욱안신방조혈청TNF-α고우REM수면박탈조（P＜0.05）；송욱안신방조혈청IL-1β교정상대조조、대평태배경대조조급REM수면박탈조무현저성차이（P＞0.05）。결론송욱안신방가능통과조절혈청세포인자TNF-α적함량종이조절수면，차통과대수면박탈대서수면적조절계이개선수면박탈소치궤체적과도능량대사。
OBJECTIVE To observe the effects of SongYuAnShenFang on serum cytokines levels of sleep depri-vation rats.METHODS The sleep deprivation in rats model was constructed with the method called “modified mul-tiple platform method”.Comparions were housed either in normal cages or in tanks with large platforms.After the treatment of SongYuAnShenFang in Sleep Deprivation Rats ,serum levels of IL-1β、TNF-αwere measured by enzyme linked immunosorbent assay.RESULTS Significant differences in serum level of TNF-αwere observed between SongYuAnShenFang group and REM sleep deprivation group ( P<0.05 ).There was no significant differences of ser-um level of IL-1βin SongYuAnShenFang group compared with the other three groups ( P>0.05 ).CONCLUSION SongYuAnShenFang in improving sleep quality may be based on its regulation of serum level of TNF-α,then adjust the excessive body metabolism caused by sleep depriveation.