CAJ | 학술논문

为鉴定市场抽检的来源不明的伪劣“羊肉卷”中的肉种成分，采用随机引物法，PCR扩增肉制品DNA，将阳性产物回收、克隆并测序，然后根据序列信息设计特异性的荧光定量引物进行再次鉴定。结果表明，获得了针对伪劣“羊肉卷”的阳性克隆，序列比对表明样品中含有北极狐或火狐肉成分，经特异性的荧光定量PCR确认样品为火狐肉。结果表明，可以应用随机引物PCR鉴定伪劣“羊肉卷”中的肉种成分。
위감정시장추검적래원불명적위렬“양육권”중적육충성분，채용수궤인물법，PCR확증육제품DNA，장양성산물회수、극륭병측서，연후근거서렬신식설계특이성적형광정량인물진행재차감정。결과표명，획득료침대위렬“양육권”적양성극륭，서렬비대표명양품중함유북겁호혹화호육성분，경특이성적형광정량PCR학인양품위화호육。결과표명，가이응용수궤인물PCR감정위렬“양육권”중적육충성분。
To identify species composition of meat and meat products sampled from market,random primer method was used for PCR amplification of meat DNA,the positive products were recovered,cloned and sequenced,and then the specific real time PCR primers were designed according to the sequence information for re-identification. Positive clones were obtained from the sliced mutton,and sequence alignment indicated that the sliced mutton sample contained Alopex lagopus or Vulpes vulpes meat ingredients. The Vulpes vulpes meat ingredient was confirmed in the sliced mutton sample by specific quantitative PCR. The results showed that the random primer PCR could be applied to the identifica-tion of species origin of meat and meat products .