CAJ | 학술논문

【目的】以含量测定为重点，开展奶牛隐性乳房炎诊断液的质量标准研究，为规范诊断液的技术标准提供参考。【方法】建立直接两相滴定法测定奶牛隐性乳房炎诊断液中的阴离子活性物含量，并以诊断液LMT为例，进行方法的准确度、精密度和专属性评价。【结果】准确度分析结果显示，RE为-0.86%（-3.88%~3.06%）；精密度分析结果显示，日内RSD为0.57%~3.14%，日间RSD为2.19%；专属性分析结果显示，LMT中的辅料成分不影响阴离子活性物的测定。直接两相滴定法测定LMT中的阴离子活性物，具备必要的准确度、精密度和专属性。【结论】因为无论是组成还是技术，LMT均能代表同类的诊断液，所以直接两相滴定法可作为奶牛隐性乳房炎诊断液的含量测定方法。
【목적】이함량측정위중점，개전내우은성유방염진단액적질량표준연구，위규범진단액적기술표준제공삼고。【방법】건립직접량상적정법측정내우은성유방염진단액중적음리자활성물함량，병이진단액LMT위례，진행방법적준학도、정밀도화전속성평개。【결과】준학도분석결과현시，RE위-0.86%（-3.88%~3.06%）；정밀도분석결과현시，일내RSD위0.57%~3.14%，일간RSD위2.19%；전속성분석결과현시，LMT중적보료성분불영향음리자활성물적측정。직접량상적정법측정LMT중적음리자활성물，구비필요적준학도、정밀도화전속성。【결론】인위무론시조성환시기술，LMT균능대표동류적진단액，소이직접량상적정법가작위내우은성유방염진단액적함량측정방법。
Objective The quality standard of diagnostic reagent for subclinical mastitis in dairy cow was studied,with particular emphasis on content determination in order to regulate the technology standard of the diagnostic reagents. Method Direct two-phase titration method was developed to determinate the anionic active matter in diagnostic reagents for subclinical mastitis in dairy cow. Taking the diagnostic reagent LMT as an example,its accuracy,precision and specificity were evaluated. Result Accuracy analysis showed that the RE was -0.86%(-3.88~3.06%). Precision analysis showed that intra-day RSD and inter-day RSD were 0.57~3.14%and 2.19%respectively. Specificity analysis showed that the result was not affected by other accessory ingredients in LMT. Direct two-phase titration method was developed to determine anionic active matter in LMT with necessary accuracy,precision and specificity. Conclusion Because LMT is representative both in composition and technology in diagnostic reagents for subclinical mastitis in dairy cows,so direct two-phase titration method could be used to content determination of the diagnostic reagents.