CAJ | 학술논문

目的：建立五子衍宗丸的高效液相色谱( HPLC)指纹图谱。方法使用 Agilent Extend C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃,以乙腈-甲醇(101)和0.4%磷酸为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长254 nm,并利用液相色谱-质谱(LC-MS)对主要色谱峰进行指认。结果根据 LC-MS 数据、对照品比对或文献参照对10个共有峰中的9个色谱峰进行了指认。用建立的对照指纹图谱测定16批五子衍宗丸,其相似度均>0.93。结论该方法精密度、稳定性、重复性良好。该方法为五子衍宗丸的全面质量评价奠定基础。
목적：건립오자연종환적고효액상색보( HPLC)지문도보。방법사용 Agilent Extend C18(250 mm×4.6 mm,5μm)색보주,주온30℃,이을정-갑순(101)화0.4%린산위류동상,제도세탈,류속1.0 mL·min-1,검측파장254 nm,병이용액상색보-질보(LC-MS)대주요색보봉진행지인。결과근거 LC-MS 수거、대조품비대혹문헌삼조대10개공유봉중적9개색보봉진행료지인。용건립적대조지문도보측정16비오자연종환,기상사도균>0.93。결론해방법정밀도、은정성、중복성량호。해방법위오자연종환적전면질량평개전정기출。
Objective To establish the high performance liquid chromatography(HPLC) fingerprint of wuzi yanzong pills. Methods HPLC was performed on Agilent Extend C18 column (250 mmí4. 6 mm,5 μm) with a gradient elution system using acetonitrile:methanol (101)-0. 4% phosphoric acid as the mobile phase. The column temperature was set at 30 ℃ and the flow rate was 1. 0 mL·min-1 . The eluate was detected at the wavelength of 254 nm. Chromatographic peaks were identified by LC-MS method. Results Nine common peaks in wuzi yanzong pill samples were identified by comparing their LC-MS data with those of reference compounds and related reference reports. The HPLC fingerprint of wuzi yanzong pills was finally developed based on the analysis of sixteen batches of samples and their similarities were above 0. 93. Conclusion This method has high precision,stability and repeatability. This study could be used for overall quality assessment of wuzi yanzong pills.