CAJ | 학술논문

目的：观察不同浓度雄黄对体外胃腺癌细胞株 SGC-7901细胞凋亡的影响。方法采用噻唑蓝(MTT)法检测0,10,20,50,80μg·mL-1浓度雄黄对胃腺癌细胞 SGC-7901增殖抑制率的影响；采用膜联蛋白吁/碘化丙啶双染色法及流式细胞仪检测0,20,50,80μg·mL-1浓度雄黄对 SGC-7901细胞凋亡的影响。结果10,20,50,80μg·mL-1浓度雄黄在24和48 h 对 SGC-7901细胞株的抑制率分别为6.15%,7.54%,42.31%,63.54%以及32.56%,46.14%,64.51%,87.52%,组间差异有统计学意义(P<0.05)。24和48 h 后细胞半数抑制浓度分别约45.23,15.34μg·mL-1。0,20,50,80μg·mL-1浓度雄黄作用24 h 后诱导的细胞早期凋亡率依次为(11.10±2.10)%,(15.31±1.30)%,(25.81±2.68)%,(43.62±8.51)%,组间差异有统计学意义(P<0.05)；晚期凋亡率依次为(1.84±0.25)%,(4.41±0.09)%,(4.37±0.14)%,(5.00±0.10)%,差异无统计学意义(P>0.05)。结论高纯度雄黄可抑制胃腺癌细胞增殖和诱导细胞早期凋亡。
목적：관찰불동농도웅황대체외위선암세포주 SGC-7901세포조망적영향。방법채용새서람(MTT)법검측0,10,20,50,80μg·mL-1농도웅황대위선암세포 SGC-7901증식억제솔적영향；채용막련단백우/전화병정쌍염색법급류식세포의검측0,20,50,80μg·mL-1농도웅황대 SGC-7901세포조망적영향。결과10,20,50,80μg·mL-1농도웅황재24화48 h 대 SGC-7901세포주적억제솔분별위6.15%,7.54%,42.31%,63.54%이급32.56%,46.14%,64.51%,87.52%,조간차이유통계학의의(P<0.05)。24화48 h 후세포반수억제농도분별약45.23,15.34μg·mL-1。0,20,50,80μg·mL-1농도웅황작용24 h 후유도적세포조기조망솔의차위(11.10±2.10)%,(15.31±1.30)%,(25.81±2.68)%,(43.62±8.51)%,조간차이유통계학의의(P<0.05)；만기조망솔의차위(1.84±0.25)%,(4.41±0.09)%,(4.37±0.14)%,(5.00±0.10)%,차이무통계학의의(P>0.05)。결론고순도웅황가억제위선암세포증식화유도세포조기조망。
Objective To observe the effect of different concentrations of realgar on the apoptosis of gastric adenocarcinoma cell Line SGC-7901. Methods MTT assay was conducted to detect the growth inhibition ratio of SGC-7901 cells treated by different concentrations of realgar (0,10,20,50 and 80 μg·mL-1 ). AnnexinⅤ/ Propidium iodide double staining method together with flow cytometry were used to detect the effect of realgar at 0,20,50 and 80 μg·mL-1 on the apoptosis of SGC-7901 cells. Results The growth inhibition ratio of SGC-7901 cells by various concentrations of high-purity realgar (10,20,50 and 80 μg·mL-1 ) was 6. 15% ,7. 54% ,42. 31% and 63. 54% ,respectively,24 h after realgar treatment. At 48 h after the treatment,the growth inhibition ratio rose to 32. 56% ,46. 14% ,64. 51% and 87. 52% ,respectively (P<0. 05). Half inhibitory concentration was 45. 23 μg·mL-1 after 24 h and 15. 34 μg·mL-1 after 48 h. After treated by various concentrations of pure realgar (0,20,50 and 80 μg·mL-1 ) for 24 h,early cell apoptosis rate was (11. 10±2. 10)% ,(15. 31±1. 30)% ,(25. 81 ±2. 68)% and (43. 62 ±8. 51)% ,respectively,significantly different among the four groups (P<0. 05). The late cell apoptosis rate in each group was (1. 84±0. 25)% ,(4. 41±0. 09)% ,(4. 37±0. 14)% and (5. 00±0. 10)% ,respectively (P>0. 05). Conclusion High purity realgar can inhibit the proliferation and induce early apoptosis of gastric adenocarcinoma SGC-7901 cells.