CAJ | 학술논문

目的：研究白芍总苷(TGP)对果糖-高脂诱导非酒精性脂肪性肝病大鼠(NAFLD)脂质浸润及纤维化的作用。方法建立果糖-高脂诱导 NAFLD 大鼠模型,用药组分别灌胃给予二甲双胍(200 mg·kg-1)、TGP 大剂量、小剂量(给予 TGP 200,100 mg·kg-1)；正常对照组和模型对照组均灌胃等量纯化水(10 mL·kg-1)。4周后,各组大鼠腹主动脉取血,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性；空腹血糖(FBG)、三酰甘油(TG)含量；检测胰岛素(INS)含量,计算胰岛素敏感指数(ISI)的变化；检测游离脂肪酸(FFA),脂肪细胞因子(Apelin-36)、内脏脂肪素(visfatin)、胶原芋(COL芋)及胶原郁(COL郁)的含量；解剖大鼠,取肝脏,测定肝组织中 TG 含量,光镜下观察肝脏组织病理学改变。结果与模型对照组比较,TGP 能有效降低 NAFLD 大鼠的 FBG、Fins 含量,提高 ISI 指数；降低血清和肝组织中 TG 含量,降低血清 ALT、AST 活性及 FAA、Apelin,visfatin,COL芋,COL郁含量,结果均差异有统计学意义(P<0.05或P<0.01)；镜检显示 TGP 能改善 NAFLD 大鼠肝脏脂质浸润及纤维化的病理学改变。结论 TGP 可能通过改善 NAFLD大鼠异常的糖脂代谢及拮抗胰岛素抵抗,有效抑制 NAFLD 大鼠的脂质浸润及纤维化。
목적：연구백작총감(TGP)대과당-고지유도비주정성지방성간병대서(NAFLD)지질침윤급섬유화적작용。방법건립과당-고지유도 NAFLD 대서모형,용약조분별관위급여이갑쌍고(200 mg·kg-1)、TGP 대제량、소제량(급여 TGP 200,100 mg·kg-1)；정상대조조화모형대조조균관위등량순화수(10 mL·kg-1)。4주후,각조대서복주동맥취혈,검측혈청병안산안기전이매(ALT)、천동안산안기전이매(AST)활성；공복혈당(FBG)、삼선감유(TG)함량；검측이도소(INS)함량,계산이도소민감지수(ISI)적변화；검측유리지방산(FFA),지방세포인자(Apelin-36)、내장지방소(visfatin)、효원우(COL우)급효원욱(COL욱)적함량；해부대서,취간장,측정간조직중 TG 함량,광경하관찰간장조직병이학개변。결과여모형대조조비교,TGP 능유효강저 NAFLD 대서적 FBG、Fins 함량,제고 ISI 지수；강저혈청화간조직중 TG 함량,강저혈청 ALT、AST 활성급 FAA、Apelin,visfatin,COL우,COL욱함량,결과균차이유통계학의의(P<0.05혹P<0.01)；경검현시 TGP 능개선 NAFLD 대서간장지질침윤급섬유화적병이학개변。결론 TGP 가능통과개선 NAFLD대서이상적당지대사급길항이도소저항,유효억제 NAFLD 대서적지질침윤급섬유화。
Objective To investigate the effect of total glucosides of paeong (TGP) on the liver lipid infiltration and fibrosis in rats with non-alcoholic fatty liver disease (NAFLD) induced by fructose and high-fat diet. Methods Fructose-high-fatty induced NAFLD rat model was established. Metformin ( MET,200 mg · kg-1 ) and TGP (200,100 mg · kg-1 ) was intragastrically given to the rats in the treatment group,TGP high dose and low dose group,respectively. Normal control group and model control group was intragastrically treated with equivalent distilled water (10 mL·kg-1 ). At the fourth week after the treatment,all the rats were sacrificed and the indices such as serum fasting blood glucose(FBG),INS,insulin sensitivity index (ISI),triglycerides(TG),apelin-36,visfatin,alanine aminotransferase(ALT),aspartate aminotransferase(AST),free fatty acid (FFA),collagen Ⅲ(COLⅢ),collagen Ⅳ(COLⅣ) were determined. Hepatic content of TG was determined and the pathological changes in the liver tissues were observed under the microscope. Results As compared with the model control group,TGP effectively decreased FBG,INS,TG in serum and liver tissues,activity of ALT and AST in serum and content of FAA,Apelin, Visfatin,COLⅢ and COLⅣ,with significant differences (P<0. 05 or P<0. 01). TGP alleviated lipid infiltration and fibrosis in rat liver tissues. Conclusion TGP can inhibit effectively lipid infiltration and fibrosis of NAFLD rats,probably through improving glucolipid metabolism and antogonizing insulin resistance.