医药导报
醫藥導報
의약도보
HERALD OF MEDICINE
2014年
10期
1291-1293
,共3页
姜辣素%黑素瘤细胞%增殖%酪氨酸酶%黑素合成
薑辣素%黑素瘤細胞%增殖%酪氨痠酶%黑素閤成
강랄소%흑소류세포%증식%락안산매%흑소합성
Gingerol%Melanoma%Proliferation%Tyrosinase%Melanin
目的：探讨姜辣素对小鼠 B16细胞黑素合成的影响。方法黑素瘤细胞分别加入12.5,25.0,50.0,100.0,200.0μmol·L-1姜辣素和阳性对照药物氢醌培养,达尔伯克改良伊格尔培养液(DMEM)作为空白对照组。采用四噻唑蓝(MTT)比色法测定细胞增殖,比色法测定酪氨酸酶活性和黑素含量。结果与空白对照组比较,含姜辣素的给药组均能够明显抑制小鼠 B16细胞增殖(P<0.05或 P<0.01),且浓度为200.0μmol·L-1时,抑制率>48%。与空白对照组比较,各浓度姜辣素均能够显著降低酪氨酸酶活性(P<0.05或 P<0.01),姜辣素的浓度为200.0μmol·L-1时,对酪氨酸酶的活性的抑制率可达50%。与空白对照组比较,各浓度的姜辣素和氢醌均能够显著降低黑素含量(P<0.05或 P<0.01),但不呈剂量依赖性,浓度>25.0μmol·L-1时,抑制黑素含量的水平基本无变化。结论姜辣素能有效抑制黑素细胞增殖和降低酪氨酸酶活性和黑素含量,从而减少黑素合成。
目的：探討薑辣素對小鼠 B16細胞黑素閤成的影響。方法黑素瘤細胞分彆加入12.5,25.0,50.0,100.0,200.0μmol·L-1薑辣素和暘性對照藥物氫醌培養,達爾伯剋改良伊格爾培養液(DMEM)作為空白對照組。採用四噻唑藍(MTT)比色法測定細胞增殖,比色法測定酪氨痠酶活性和黑素含量。結果與空白對照組比較,含薑辣素的給藥組均能夠明顯抑製小鼠 B16細胞增殖(P<0.05或 P<0.01),且濃度為200.0μmol·L-1時,抑製率>48%。與空白對照組比較,各濃度薑辣素均能夠顯著降低酪氨痠酶活性(P<0.05或 P<0.01),薑辣素的濃度為200.0μmol·L-1時,對酪氨痠酶的活性的抑製率可達50%。與空白對照組比較,各濃度的薑辣素和氫醌均能夠顯著降低黑素含量(P<0.05或 P<0.01),但不呈劑量依賴性,濃度>25.0μmol·L-1時,抑製黑素含量的水平基本無變化。結論薑辣素能有效抑製黑素細胞增殖和降低酪氨痠酶活性和黑素含量,從而減少黑素閤成。
목적：탐토강랄소대소서 B16세포흑소합성적영향。방법흑소류세포분별가입12.5,25.0,50.0,100.0,200.0μmol·L-1강랄소화양성대조약물경곤배양,체이백극개량이격이배양액(DMEM)작위공백대조조。채용사새서람(MTT)비색법측정세포증식,비색법측정락안산매활성화흑소함량。결과여공백대조조비교,함강랄소적급약조균능구명현억제소서 B16세포증식(P<0.05혹 P<0.01),차농도위200.0μmol·L-1시,억제솔>48%。여공백대조조비교,각농도강랄소균능구현저강저락안산매활성(P<0.05혹 P<0.01),강랄소적농도위200.0μmol·L-1시,대락안산매적활성적억제솔가체50%。여공백대조조비교,각농도적강랄소화경곤균능구현저강저흑소함량(P<0.05혹 P<0.01),단불정제량의뢰성,농도>25.0μmol·L-1시,억제흑소함량적수평기본무변화。결론강랄소능유효억제흑소세포증식화강저락안산매활성화흑소함량,종이감소흑소합성。
Objective To study the effects of the gingerol on the melanogenesis in melanoma B16 cells. Methods Melanoma cells were cultured with gingerol at 12. 5, 25. 0, 50. 0, 100. 0, 200. 0 μmol · L-1 and positive control drug hydroquinone,respectively,using Dulbecco's modified eagle's medium(DMEM) as the blank control group. The cell proliferation was measured by methyl thiazolyltet tetrazolium ( MTT) colorimetric assay. The tyrosinase activity and melanin content were measured by colorimetry assay. Results Gingerol at different concentrations had inhibitory effect on B16 cell proliferation compared with the blank control group ( P < 0. 05 or P < 0. 01), the inhibition rate being more than 48% at the dosage of 200. 0 μmol·L-1 . Tyrosinase activity was inhibited significantly compared with blank control group(P<0. 05 or P<0. 01),the inhibition rate being up to 50% at the dosage of 200 μmol·L-1 . Melanin content was also decreased at all levels of gingerol compared with blank control group(P<0. 05 or P<0. 01),but not in a dose-dependent manner. The inhibition rate of melanin content reached the plateau at gingerol levels greater than 25. 0 μmol · L-1 . Conclusion Gingerol can inhibit the cell proliferation,tyrosinase activity and decrease melanin synthesis in certain range of concentrations.
