CAJ | 학술논문

目的：体外检测多肽 P161联合顺铂(DDP)对多种肿瘤细胞增殖的抑制效应。方法采用四甲基偶氮唑盐(MTT)法检测不同浓度 DDP、P161单用及联合用药对人肝癌 HepG2、人结肠癌 HT29、人前列腺癌 IE8、人胰腺癌 Panc-1及小鼠乳腺癌 MA-782的生长抑制率。结果 DDP 和 P161对多种肿瘤细胞均有剂量依赖性的抗增殖作用。联合给药组表现为协同效应，与 DDP 组比较差异有统计学意义(P<0.05)，在相同的抑癌效果下可降低 DDP 的使用量。且在相同浓度梯度下，联合用药组对 Panc-1的抑制效果较差，而对 MA-782效果最好。结论 P161可以促进肿瘤细胞对 DDP 的敏感性，二者联合用药可降低 DDP 有效治疗浓度。
목적：체외검측다태 P161연합순박(DDP)대다충종류세포증식적억제효응。방법채용사갑기우담서염(MTT)법검측불동농도 DDP、P161단용급연합용약대인간암 HepG2、인결장암 HT29、인전렬선암 IE8、인이선암 Panc-1급소서유선암 MA-782적생장억제솔。결과 DDP 화 P161대다충종류세포균유제량의뢰성적항증식작용。연합급약조표현위협동효응，여 DDP 조비교차이유통계학의의(P<0.05)，재상동적억암효과하가강저 DDP 적사용량。차재상동농도제도하，연합용약조대 Panc-1적억제효과교차，이대 MA-782효과최호。결론 P161가이촉진종류세포대 DDP 적민감성，이자연합용약가강저 DDP 유효치료농도。
Objective In vitro detection of anti-proliferative effects of P161 combined with cisplatin ( DDP) on multiple cancer cells. Methods Growth inhibition rates of HepG2, HT29, IE8, Panc-1 and MA-782 treated by different concentrations of DDP,P161 and P161 combined with DDP were determined by MTT assay. Results DDP and P161 dose-dependently inhibited proliferation of multiple tumor cells. A synergistic effect was found in DDP combined with P161 and there was a significant difference in the effect between DDP combined with P161 and DDP alone (P<0. 05). DDP dose could be decreased to reach the same inhibitory effect. In the same concentration gradient of DDP combined with P161,the inhibition rate of Panc-1 was low and that of MA-782 was high. Conclusion P161 can increase the sensitivity of tumor cells to DDP. The combination of P161 and DDP can reduce the effective therapeutic concentration of DDP.