CAJ | 학술논문

目的：观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体功能的影响，比较其单独及协同作用，探讨耐力训练及限食的线粒体机制。方法：32只17月龄雄性SD大鼠分为4组：安静组（Control，C）、限食组（Caloric-Restricted，CR）、运动组（Exercise，E）和限食加运动组（Caloric-restricted and Exercise，E＋CR），训练方式为跑台运动，中等运动强度（64％VO2max，15m/min，60分钟/天，每周5天），限食摄入的标准为正常摄入组的60％，共训练及限食12周，相同月龄对照组正常饲养。12周后于末次训练后取大鼠骨骼肌进行线粒体呼吸功能测定。结果：态3呼吸CR组和CR＋E组显著降低，E组明显增高（P＜0．05）；态4呼吸CR组显著降低（P＜0．05），E组和CR＋E无明显变化；呼吸控制比CR组和CR＋E组显著降低，E组明显增高（P＜0．05）；ATP合成活力E组显著增加（P＜0．05），而CR组和CR＋E组无明显变化，结论：耐力训练改善了大鼠骨骼肌线粒体呼吸链的电子传递效率，提高了线粒体呼吸功能，增强了氧化磷酸化能力，限食对能量需求较小，ATP生成减少，在低消耗水平下对线粒体呼吸功能有着良性影响。
목적：관찰장기내력훈련급한식대노령대서골격기선립체공능적영향，비교기단독급협동작용，탐토내력훈련급한식적선립체궤제。방법：32지17월령웅성SD대서분위4조：안정조（Control，C）、한식조（Caloric-Restricted，CR）、운동조（Exercise，E）화한식가운동조（Caloric-restricted and Exercise，E＋CR），훈련방식위포태운동，중등운동강도（64％VO2max，15m/min，60분종/천，매주5천），한식섭입적표준위정상섭입조적60％，공훈련급한식12주，상동월령대조조정상사양。12주후우말차훈련후취대서골격기진행선립체호흡공능측정。결과：태3호흡CR조화CR＋E조현저강저，E조명현증고（P＜0．05）；태4호흡CR조현저강저（P＜0．05），E조화CR＋E무명현변화；호흡공제비CR조화CR＋E조현저강저，E조명현증고（P＜0．05）；ATP합성활력E조현저증가（P＜0．05），이CR조화CR＋E조무명현변화，결론：내력훈련개선료대서골격기선립체호흡련적전자전체효솔，제고료선립체호흡공능，증강료양화린산화능력，한식대능량수구교소，ATP생성감소，재저소모수평하대선립체호흡공능유착량성영향。
Objective:we observe the long -term effects of endurance training and diet restriction on skeletal muscle mitochondrial function in aging rats,comparing their individual and synergistic effects of endurance training and diet restriction,investigating mitochondrial mechanism.Method32 adult male 17month old SD rats were divided into 4 equal groupcontrol,caloric -restricted,exercise,exercise and caloric -restricted. Exercise and exercise and caloric-restricted groups were trained for 12months by running on a treadmill (64%VO2max;15 m/min,60 min/day;5 days/week ),while caloric -restricted group were maintained on 60%caloric intake of normal caloric intake for 12months.Measuring mitochondrial respiratory function in rat skeletal muscle after the last training of 12 weeks.ResultState3respirationCRgroup and CR +E group was significantly lower,E group were significantly higher(P <0.05);State4respirationCRgroup was significantly reduce(P <0.05),E group and CR +E no significant change;Respiratory control ratio was significantly lower in the CR group and CR +E group,E significantly increased(P<0.05 );ATP synthesis activity was significant increase in group E (P <0.05 ),while the CR group and CR +E group had no significant change.ConclusionEndurance training improves the efficiency of electron transfer in rat skeletal muscle mitochondrial respiratory chain,improving mitochondrial respiratory function,and enhancing the ability of oxidative phosphorylation, reducing the demand for energy restricted diet,reducing the efficiency of mitochondrial oxidative phosphorylation.