CAJ | 학술논문

背景：软骨进行性破坏为晚期骨关节炎的特征性病变，骨关节炎相关因子透明质酸、骨桥蛋白、CD44在骨关节炎软骨中表达增加。 　　目的：通过透明质酸干预体外培养的人膝骨关节炎软骨细胞，探讨透明质酸对人膝骨关节炎软骨细胞 CD44与骨桥蛋白表达的影响。 　　方法：将软骨标本进行体外培养获取纯化的软骨细胞，分为3组：空白对照组、透明质酸干预组(100 mg/L)和透明质酸酶干预组(200 mg/L)。培养48 h后，采用Real-time Q PCR检测软骨细胞骨桥蛋白mRNA，CD44 mRNA表达水平。用SPSS 17.0统计软件包分析骨桥蛋白mRNA和CD44 mRNA经透明质酸干预前后表达的差异。 　　结果与结论：透明质酸组的骨桥蛋白 mRNA表达水平较空白组高，透明质酸酶组的骨桥蛋白 mRNA表达水平较空白组低；透明质酸组及透明质酸酶组的CD44 mRNA表达水平均较空白组低。结果提示透明质酸可以上调骨关节炎软骨细胞骨桥蛋白的表达；透明质酸在骨关节炎软骨细胞内对 CD44表达的影响具有双相性，其影响结果可能与透明质酸的相对分子质量有关。
배경：연골진행성파배위만기골관절염적특정성병변，골관절염상관인자투명질산、골교단백、CD44재골관절염연골중표체증가。 　　목적：통과투명질산간예체외배양적인슬골관절염연골세포，탐토투명질산대인슬골관절염연골세포 CD44여골교단백표체적영향。 　　방법：장연골표본진행체외배양획취순화적연골세포，분위3조：공백대조조、투명질산간예조(100 mg/L)화투명질산매간예조(200 mg/L)。배양48 h후，채용Real-time Q PCR검측연골세포골교단백mRNA，CD44 mRNA표체수평。용SPSS 17.0통계연건포분석골교단백mRNA화CD44 mRNA경투명질산간예전후표체적차이。 　　결과여결론：투명질산조적골교단백 mRNA표체수평교공백조고，투명질산매조적골교단백 mRNA표체수평교공백조저；투명질산조급투명질산매조적CD44 mRNA표체수평균교공백조저。결과제시투명질산가이상조골관절염연골세포골교단백적표체；투명질산재골관절염연골세포내대 CD44표체적영향구유쌍상성，기영향결과가능여투명질산적상대분자질량유관。
BACKGROUND:Progressive fracture of the cartilage is considered the characteristic lesion in later osteoarthritis, the expression of osteoarthritis-related factors such as hyaluronic acid, osteopontin and CD44 in osteoarthritic cartilage is increased. OBJECTIVE:To investigate the effect of hyaluronic acid on the expression of osteopontin mRNA and CD44 mRNA of chondrocytes in the in vitro cultured chondrocytes of patients with knee osteoarthritis. METHODThe cartilage samples obtained from osteoarthritic patients were cultured and purified into acquire chondrocytes in vitro, and the cells were divided into three groupblank control group, hyaluronic acid (100 mg/mL) group and hyaluronidase (200 mg/mL) group. After 48 hours of cellculture, real-time quantitative polymerase chain reaction assay was used to detect the expression of CD44 mRNA and osteopontin mRNA. The difference of the expression levels before and after the intervention of hyaluronic acid was compared and analyzed using SPSS 17.0 software. RESULTS AND CONCLUSION:Compared with the blank control group, hyaluronic acid (100 mg/mL) upregulated osteopontin mRNA expression in the chondrocytes, hyaluronidase (200 mg/mL) also reduced osteopontin mRNA expression in the chondrocytes. The CD44 mRNA expression in the chondrocytes of hyaluronic acid (100 mg/mL) group and hyaluronidase (200 mg/mL) group was lower than that in the blank control group. Hyaluronic acid can upregulate the expression of the osteopontin mRNA expression in the osteoarthritic chondrocytes;the biphasic effects of hyaluronic acid on CD44 mRNA expression in osteoarthritic chondrocytes might be associated with the molecule weight of hyaluronic acid.