中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
38期
6160-6164
,共5页
组织构建%组织工程%亚甲蓝注射液%脊髓%神经节%安全性
組織構建%組織工程%亞甲藍註射液%脊髓%神經節%安全性
조직구건%조직공정%아갑람주사액%척수%신경절%안전성
spinal cord%methylene blue%ganglia
背景：亚甲蓝可阻碍感觉神经的异常疼痛传导，其机制是阻断缓激肽诱导的痛觉过敏，消除局部组织炎症引起的痛觉过敏反应。<br> 目的：观察亚甲蓝溶液对大鼠腰椎脊髓及脊神经节功能的影响，明确亚甲蓝治疗椎间盘源性腰痛的安全性。方法：将120只Wistar大鼠随机分为5组，每组24只。暴露大鼠腰节段硬膜，亚甲蓝0.2 mL组、亚甲蓝1 mL组、亚甲蓝2 mL组分别于大鼠硬膜外注入相应量的亚甲蓝；生理盐水组大鼠硬膜外注入1 mL生理盐水；空白对照组不进行此步操作。分别在注入后30 min，2 h，24 h，72 h每组分别随机对6只大鼠进行灌流固定，并切除相应节段脊髓及神经节，行苏木精-伊红染色，在光镜下观察对比组织结构改变。<br> 结果与结论：亚甲蓝各组大鼠注入后30 min，2 h，24 h，72 h时间脊髓及神经根苏木精-伊红染色，显示脊髓背侧面结构完整，白质与灰质分界清晰，白质中神经纤维致密，纤维间有圆形或卵圆形的神经胶质细胞核；灰质后角神经纤维致密；神经元细胞，胞核圆形染色浅，核仁明显；细胞群间有成束的神经纤维。亚甲蓝各组大鼠腰椎脊髓及脊神经节与生理盐水组及空白对照组比较均无明显的组织结构变化。结果表明硬膜外注入1%亚甲蓝对脊髓及脊神经结构无明显影响。
揹景：亞甲藍可阻礙感覺神經的異常疼痛傳導，其機製是阻斷緩激肽誘導的痛覺過敏，消除跼部組織炎癥引起的痛覺過敏反應。<br> 目的：觀察亞甲藍溶液對大鼠腰椎脊髓及脊神經節功能的影響，明確亞甲藍治療椎間盤源性腰痛的安全性。方法：將120隻Wistar大鼠隨機分為5組，每組24隻。暴露大鼠腰節段硬膜，亞甲藍0.2 mL組、亞甲藍1 mL組、亞甲藍2 mL組分彆于大鼠硬膜外註入相應量的亞甲藍；生理鹽水組大鼠硬膜外註入1 mL生理鹽水；空白對照組不進行此步操作。分彆在註入後30 min，2 h，24 h，72 h每組分彆隨機對6隻大鼠進行灌流固定，併切除相應節段脊髓及神經節，行囌木精-伊紅染色，在光鏡下觀察對比組織結構改變。<br> 結果與結論：亞甲藍各組大鼠註入後30 min，2 h，24 h，72 h時間脊髓及神經根囌木精-伊紅染色，顯示脊髓揹側麵結構完整，白質與灰質分界清晰，白質中神經纖維緻密，纖維間有圓形或卵圓形的神經膠質細胞覈；灰質後角神經纖維緻密；神經元細胞，胞覈圓形染色淺，覈仁明顯；細胞群間有成束的神經纖維。亞甲藍各組大鼠腰椎脊髓及脊神經節與生理鹽水組及空白對照組比較均無明顯的組織結構變化。結果錶明硬膜外註入1%亞甲藍對脊髓及脊神經結構無明顯影響。
배경：아갑람가조애감각신경적이상동통전도，기궤제시조단완격태유도적통각과민，소제국부조직염증인기적통각과민반응。<br> 목적：관찰아갑람용액대대서요추척수급척신경절공능적영향，명학아갑람치료추간반원성요통적안전성。방법：장120지Wistar대서수궤분위5조，매조24지。폭로대서요절단경막，아갑람0.2 mL조、아갑람1 mL조、아갑람2 mL조분별우대서경막외주입상응량적아갑람；생리염수조대서경막외주입1 mL생리염수；공백대조조불진행차보조작。분별재주입후30 min，2 h，24 h，72 h매조분별수궤대6지대서진행관류고정，병절제상응절단척수급신경절，행소목정-이홍염색，재광경하관찰대비조직결구개변。<br> 결과여결론：아갑람각조대서주입후30 min，2 h，24 h，72 h시간척수급신경근소목정-이홍염색，현시척수배측면결구완정，백질여회질분계청석，백질중신경섬유치밀，섬유간유원형혹란원형적신경효질세포핵；회질후각신경섬유치밀；신경원세포，포핵원형염색천，핵인명현；세포군간유성속적신경섬유。아갑람각조대서요추척수급척신경절여생리염수조급공백대조조비교균무명현적조직결구변화。결과표명경막외주입1%아갑람대척수급척신경결구무명현영향。
BACKGROUND:Methylene blue can hinder abnormal pain conduction via the sensory nerve, and its mechanism is to block bradykinin-induced hyperalgesia and eliminate pain caused by local tissue inflammation. <br> OBJECTIVE:To observe the influence of methylene blue solution on the lumbar spinal cord and spinal ganglia function of rats, and to investigate whether methylene blue is safe for treating discogenic low back pain. <br> METHODTotal y 120 Wistar rats were randomly divided into five groupthree experimental groups, a saline control group and a blank control group, n=24 in each group. Lumbar segmental dura was exposed in rats. In the three experimental groups, 0.2, 1, and 2 mL methylene blue were injected epidural y, respectively. The saline control group was subjected to the epidural injection of 1 mL saline. In the blank control group, there was no treatment. Six rats from each group were selected randomly and perfused at 30 minutes, 2 hours, 24 hours, 72 hours after injection, respectively. Then, the corresponding segments of the spinal cord and ganglions were removed. Hematoxylin eosin staining was used for comparing histological and structural changes under light microscope. <br> RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that at 30 minutes, 2 hours, 24 hours and 72 hours after injection of methylene blue, the spinal dorsal side exhibited the structural integrity, clear boundaries between the white matter and gray matter, dense nerve fibers in the white matter, and round or oval nuclei of glial cells among fibers;dense nerve fibers in the posterior horn of gray matter;light-colored neuronal nuclei with prominent nucleoli;a bundle of nerve fibers among cellpopulations. There was no significant change in tissue structure of lumbar spinal cord and spinal ganglia between the experimental groups and the saline control group or between the experimental groups and the blank control group. Thus, the epidural injection of 1%methylene blue has no significant effect on the spinal cord and spinal nerve structures.
