中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
38期
6099-6109
,共11页
组织构建%骨组织工程%骨形态发生蛋白%富血小板血浆%颌骨%β-磷酸三钙
組織構建%骨組織工程%骨形態髮生蛋白%富血小闆血漿%頜骨%β-燐痠三鈣
조직구건%골조직공정%골형태발생단백%부혈소판혈장%합골%β-린산삼개
maxil a%bone morphogenetic proteins%platelet-rich plasma%calcium phosphates
背景:研究表明骨形态发生蛋白在骨骼发育中的起到关键性作用;文献报道单独的富血小板血浆在动物或者临床试验中并不具备明显促进移植骨组织愈合的作用。<br> 目的:验证富血小板血浆协同骨形态发生蛋白4促骨缺损区的成骨作用。<br> 方法:24只新西兰大白兔构建上颌骨骨缺损模型,随机分为4组,每组6只。①空白对照组建模后不做特殊处理。②β-磷酸三钙+Bio-gide生物膜组为β-磷酸三钙0.1 g+Bio-gide生物膜1张。③富血小板血浆组为β-磷酸三钙0.1 g+Bio-gide生物膜1张+富血小板血浆1 mL。④富血小板血浆+骨形态发生蛋白4组为β-磷酸三钙0.1 g+Bio-gide生物膜1张+富血小板血浆1 mL+5μg骨形态发生蛋白4。于造模后4,8,12周行大体观察、组织学观察及图像分析各组骨缺损区新生骨生成情况。<br> 结果与结论:术后4周,富血小板血浆组、富血小板血浆+骨形态发生蛋白4组骨缺损区新生骨以及新生血管均多于β-磷酸三钙+Bio-gide组(P<0.01);β-磷酸三钙+Bio-gide组新成骨多于空白对照组(P<0.01)。术后4,8,12周,富血小板血浆+骨形态发生蛋白4组骨缺损区新生骨均多于β-磷酸三钙+Bio-gide组、富血小板血浆组两组骨缺损区(P<0.01),β-磷酸三钙+Bio-gide组和富血小板血浆组均多于空白对照组(P<0.01)。结果表明以β-磷酸三钙为支架,富血小板血浆复合骨形态发生蛋白可明显促进骨缺损的愈合。
揹景:研究錶明骨形態髮生蛋白在骨骼髮育中的起到關鍵性作用;文獻報道單獨的富血小闆血漿在動物或者臨床試驗中併不具備明顯促進移植骨組織愈閤的作用。<br> 目的:驗證富血小闆血漿協同骨形態髮生蛋白4促骨缺損區的成骨作用。<br> 方法:24隻新西蘭大白兔構建上頜骨骨缺損模型,隨機分為4組,每組6隻。①空白對照組建模後不做特殊處理。②β-燐痠三鈣+Bio-gide生物膜組為β-燐痠三鈣0.1 g+Bio-gide生物膜1張。③富血小闆血漿組為β-燐痠三鈣0.1 g+Bio-gide生物膜1張+富血小闆血漿1 mL。④富血小闆血漿+骨形態髮生蛋白4組為β-燐痠三鈣0.1 g+Bio-gide生物膜1張+富血小闆血漿1 mL+5μg骨形態髮生蛋白4。于造模後4,8,12週行大體觀察、組織學觀察及圖像分析各組骨缺損區新生骨生成情況。<br> 結果與結論:術後4週,富血小闆血漿組、富血小闆血漿+骨形態髮生蛋白4組骨缺損區新生骨以及新生血管均多于β-燐痠三鈣+Bio-gide組(P<0.01);β-燐痠三鈣+Bio-gide組新成骨多于空白對照組(P<0.01)。術後4,8,12週,富血小闆血漿+骨形態髮生蛋白4組骨缺損區新生骨均多于β-燐痠三鈣+Bio-gide組、富血小闆血漿組兩組骨缺損區(P<0.01),β-燐痠三鈣+Bio-gide組和富血小闆血漿組均多于空白對照組(P<0.01)。結果錶明以β-燐痠三鈣為支架,富血小闆血漿複閤骨形態髮生蛋白可明顯促進骨缺損的愈閤。
배경:연구표명골형태발생단백재골격발육중적기도관건성작용;문헌보도단독적부혈소판혈장재동물혹자림상시험중병불구비명현촉진이식골조직유합적작용。<br> 목적:험증부혈소판혈장협동골형태발생단백4촉골결손구적성골작용。<br> 방법:24지신서란대백토구건상합골골결손모형,수궤분위4조,매조6지。①공백대조조건모후불주특수처리。②β-린산삼개+Bio-gide생물막조위β-린산삼개0.1 g+Bio-gide생물막1장。③부혈소판혈장조위β-린산삼개0.1 g+Bio-gide생물막1장+부혈소판혈장1 mL。④부혈소판혈장+골형태발생단백4조위β-린산삼개0.1 g+Bio-gide생물막1장+부혈소판혈장1 mL+5μg골형태발생단백4。우조모후4,8,12주행대체관찰、조직학관찰급도상분석각조골결손구신생골생성정황。<br> 결과여결론:술후4주,부혈소판혈장조、부혈소판혈장+골형태발생단백4조골결손구신생골이급신생혈관균다우β-린산삼개+Bio-gide조(P<0.01);β-린산삼개+Bio-gide조신성골다우공백대조조(P<0.01)。술후4,8,12주,부혈소판혈장+골형태발생단백4조골결손구신생골균다우β-린산삼개+Bio-gide조、부혈소판혈장조량조골결손구(P<0.01),β-린산삼개+Bio-gide조화부혈소판혈장조균다우공백대조조(P<0.01)。결과표명이β-린산삼개위지가,부혈소판혈장복합골형태발생단백가명현촉진골결손적유합。
BACKGROUND:Studies have shown that bone morphogenetic proteins play a key role in skeletal development. Platelet-rich plasma alone in animal or clinical trials cannot significantly promote bone graft healing. <br> OBJECTIVE:To investigate the osteogenesis effect of bone morphogenetic protein-4 compounded with platelet-rich plasma the bone defect area. <br> METHODTwenty-four New Zealand white rabbits were selected to establish maxil ary bone defect models, and then were randomly divided into four groups, six rats in each group. Group A was blank control group;Group B wasβ-tricalcium phosphate (0.1 g)+Bio-gide group;group C wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL) group;and group D wasβ-tricalcium phosphate (0.1 g)+Bio-gide+platelet-rich plasma (1 mL)+bone morphogenetic protein-4 (5μg). Gross observation, histological observation and imaging analysis were performed for analysis of new bone formation at weeks 4, 8, 12 after modeling. <br> RESULTS AND CONCLUSION:After 4 weeks, group D had more new bone and vessels formed in the bone defect area than the group B (P<0.01);however, the amount of new bone was higher in the group B than the group A (P<0.01). After 4, 8, 12 weeks, the amount of new bone in the bone defect area was higher in the group D than the groups B and C (P<0.01), and lowest in the group A (P<0.01). In theβ-tricalcium phosphate scaffold, platelet-rich plasma combined with bone morphogenetic protein can significantly promote the healing of bone defects.