中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
38期
6074-6078
,共5页
组织构建%组织工程%血管平滑肌细胞%p21基因%甲基化%动脉粥样硬化%细胞周期蛋白
組織構建%組織工程%血管平滑肌細胞%p21基因%甲基化%動脈粥樣硬化%細胞週期蛋白
조직구건%조직공정%혈관평활기세포%p21기인%갑기화%동맥죽양경화%세포주기단백
genes%methylation%atherosclerosis%cyclins
背景：血管平滑肌细胞增殖、迁移及表型改变是动脉粥样硬化发生的中心环节，一系列相关基因的甲基化参与该过程。<br> 目的：观察血管平滑肌细胞p21基因启动子甲基化对其增殖活性的影响。<br> 方法：采用组织贴块法培养人脐血管平滑肌细胞，给予不同质量浓度氧化低密度脂蛋白(0，10，20，40 mg/L)孵育24 h，甲基化特异PCR检测p21基因启动子区CpG岛甲基化程度，反转录PCR检测p21 mRNA表达， MTT比色法测定血管平滑肌细胞增殖活性。<br> 结果与结论：氧化低密度脂蛋白呈浓度依赖性促进p21启动子甲基化和降低p21 mRNA表达，同时平滑细胞增殖活性增高。提示氧化低密度脂蛋白可以通过p21基因甲基化促进血管平滑肌细胞增殖，p21基因甲基化可能在动脉粥样硬化发生中起到一定作用。
揹景：血管平滑肌細胞增殖、遷移及錶型改變是動脈粥樣硬化髮生的中心環節，一繫列相關基因的甲基化參與該過程。<br> 目的：觀察血管平滑肌細胞p21基因啟動子甲基化對其增殖活性的影響。<br> 方法：採用組織貼塊法培養人臍血管平滑肌細胞，給予不同質量濃度氧化低密度脂蛋白(0，10，20，40 mg/L)孵育24 h，甲基化特異PCR檢測p21基因啟動子區CpG島甲基化程度，反轉錄PCR檢測p21 mRNA錶達， MTT比色法測定血管平滑肌細胞增殖活性。<br> 結果與結論：氧化低密度脂蛋白呈濃度依賴性促進p21啟動子甲基化和降低p21 mRNA錶達，同時平滑細胞增殖活性增高。提示氧化低密度脂蛋白可以通過p21基因甲基化促進血管平滑肌細胞增殖，p21基因甲基化可能在動脈粥樣硬化髮生中起到一定作用。
배경：혈관평활기세포증식、천이급표형개변시동맥죽양경화발생적중심배절，일계렬상관기인적갑기화삼여해과정。<br> 목적：관찰혈관평활기세포p21기인계동자갑기화대기증식활성적영향。<br> 방법：채용조직첩괴법배양인제혈관평활기세포，급여불동질량농도양화저밀도지단백(0，10，20，40 mg/L)부육24 h，갑기화특이PCR검측p21기인계동자구CpG도갑기화정도，반전록PCR검측p21 mRNA표체， MTT비색법측정혈관평활기세포증식활성。<br> 결과여결론：양화저밀도지단백정농도의뢰성촉진p21계동자갑기화화강저p21 mRNA표체，동시평활세포증식활성증고。제시양화저밀도지단백가이통과p21기인갑기화촉진혈관평활기세포증식，p21기인갑기화가능재동맥죽양경화발생중기도일정작용。
BACKGROUND:Proliferation, migration and phenotypic changes of vascular smooth muscle cells is the core of the occurrence of atherosclerosis, and a series of related genes via methylation are involved in the process. <br> OBJECTIVE:To investigate the effects of oxidized low density lipoprotein (ox-LDL) on DNA methylation in the promoter region of the p21 gene and its potential mechanism in the pathogenesis of atherosclerosis. <br> METHODCultured human vascular smooth muscle cells were treated with different concentrations of ox-LDL (0, 10, 20, 40 mg/L) for 24 hours. The degree of DNA methylation was assayed by methylation-specific polymerase chain reaction, the expression of p21 mRNA was measured by reverse transcriptional polymerase chain reaction and the proliferative activity of vascular smooth muscle cells was determined by the MTT assay. <br> RESULTS AND CONCLUSION:The ox-LDL treatment resulted in a promotion in the methylation in the promoter region of the p21 gene and a decrease in mRNA expression with a concentration-dependent manner;it also induced a dose-dependent promoting effect on vascular smooth muscle cellproliferation. The atherogenic mechanism of ox-LDL might promote vascular smooth muscle cellproliferation by the hypermethylation of the p21 gene that may lead to the occurrence and development of atherosclerosis.
