CAJ | 학술논문

以雪落樱（ Cerasus xueluoensis）叶片为外植体，研究灭菌方式、不同激素配比组合及含糖量等因素对叶片再生的影响。结果表明，以春季充分展开的嫩叶为外植体，用0．1％ HgCl2灭菌7 min效果较理想。筛选出MS为最适的基本培养基，并在培养基中添加20 mg/L Vc溶液，能有效缓解愈伤组织继代过程中的褐变状况；愈伤组织增殖以MS＋6-BA（1．0 mg/L）＋NAA（0．5 mg/L）＋Vc（20 mg/L）为宜，增殖快速且生长良好；在MS＋6-BA（2．0 mg/L）＋NAA（0．1 mg/L）＋TDZ（2．0 mg/L）＋Vc（20 mg/L）培养基中愈伤组织分化最理想，不定芽生长健壮；将分化健壮的不定芽接种到3/4MS＋NAA（0．1 mg/L）＋蔗糖（15 g/L）的培养基中生根，27 d后生根率达100％。
이설락앵（ Cerasus xueluoensis）협편위외식체，연구멸균방식、불동격소배비조합급함당량등인소대협편재생적영향。결과표명，이춘계충분전개적눈협위외식체，용0．1％ HgCl2멸균7 min효과교이상。사선출MS위최괄적기본배양기，병재배양기중첨가20 mg/L Vc용액，능유효완해유상조직계대과정중적갈변상황；유상조직증식이MS＋6-BA（1．0 mg/L）＋NAA（0．5 mg/L）＋Vc（20 mg/L）위의，증식쾌속차생장량호；재MS＋6-BA（2．0 mg/L）＋NAA（0．1 mg/L）＋TDZ（2．0 mg/L）＋Vc（20 mg/L）배양기중유상조직분화최이상，불정아생장건장；장분화건장적불정아접충도3/4MS＋NAA（0．1 mg/L）＋자당（15 g/L）적배양기중생근，27 d후생근솔체100％。
A plant regeneration system via adventitious shoot formation from leaf explants of Cerasus xueluoensis was es-tablished. Several factors such as explant sterilizing time, hormone concentration and concentration of sucrose and so on were explored to optimize the regeneration system in virto. The result showed that the fully unfold leaves were selected as the most appropriate explant in spring. And the best basic medium is MS. With 6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc( 20 mg/L) was the optimum medium for callus proliferation. And 20 mg/L Vc was best to inhibit C. xueluoensis cal-lus from browning. MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L) was the optimum medium for shoot differentiation. And 3/4MS+NAA(0.1 mg/L)+sucrose (15 g/L) was the optimum medium for root differentiation. The rooting percentage was 100% after 27 days.