南京林业大学学报（自然科学版）
南京林業大學學報（自然科學版）
남경임업대학학보（자연과학판）
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2014年
z1期
7-12
,共6页
张洪伟%段一凡%李稚%王贤荣
張洪偉%段一凡%李稚%王賢榮
장홍위%단일범%리치%왕현영
桂花%花粉活力%贮藏方法
桂花%花粉活力%貯藏方法
계화%화분활력%저장방법
Osmanthus fragrans (Thunb.) Lour.%pollen vitality%storage method
研究不同贮藏方法对桂花花粉活力的影响，探索桂花花粉保存的适宜条件。以4个品种群中的9种桂花品种为试验材料，对其进行常温、低温、低温冷冻和超低温冷冻贮藏，并对不同贮藏时间内的花粉活力进行检测。结果表明：桂花花粉在10％的蔗糖、0．01％硼酸和1％的琼脂糖组成的固体培养基上，25℃恒温培养24 h后花粉基本充分萌发，可用于萌发率的统计；光照和黑暗对花粉萌发无显著影响。不同贮藏条件下花粉保存效果差异显著，室温条件（15～20℃）下，大多数品种花粉寿命为15～20 d；低温贮藏（4℃）花粉活力可延长至50 d，低温冷冻（-20℃）和超低温冷冻（-70℃）贮藏100 d后大多数桂花品种的花粉萌发率差异不显著。在低温冷冻和超低温冷冻条件下桂花花粉活力下降较慢，可用于花粉较长时间的保存。
研究不同貯藏方法對桂花花粉活力的影響，探索桂花花粉保存的適宜條件。以4箇品種群中的9種桂花品種為試驗材料，對其進行常溫、低溫、低溫冷凍和超低溫冷凍貯藏，併對不同貯藏時間內的花粉活力進行檢測。結果錶明：桂花花粉在10％的蔗糖、0．01％硼痠和1％的瓊脂糖組成的固體培養基上，25℃恆溫培養24 h後花粉基本充分萌髮，可用于萌髮率的統計；光照和黑暗對花粉萌髮無顯著影響。不同貯藏條件下花粉保存效果差異顯著，室溫條件（15～20℃）下，大多數品種花粉壽命為15～20 d；低溫貯藏（4℃）花粉活力可延長至50 d，低溫冷凍（-20℃）和超低溫冷凍（-70℃）貯藏100 d後大多數桂花品種的花粉萌髮率差異不顯著。在低溫冷凍和超低溫冷凍條件下桂花花粉活力下降較慢，可用于花粉較長時間的保存。
연구불동저장방법대계화화분활력적영향，탐색계화화분보존적괄의조건。이4개품충군중적9충계화품충위시험재료，대기진행상온、저온、저온냉동화초저온냉동저장，병대불동저장시간내적화분활력진행검측。결과표명：계화화분재10％적자당、0．01％붕산화1％적경지당조성적고체배양기상，25℃항온배양24 h후화분기본충분맹발，가용우맹발솔적통계；광조화흑암대화분맹발무현저영향。불동저장조건하화분보존효과차이현저，실온조건（15～20℃）하，대다수품충화분수명위15～20 d；저온저장（4℃）화분활력가연장지50 d，저온냉동（-20℃）화초저온냉동（-70℃）저장100 d후대다수계화품충적화분맹발솔차이불현저。재저온냉동화초저온냉동조건하계화화분활력하강교만，가용우화분교장시간적보존。
The aim was to research the effects of different storage methods on pollen vitality of Osmanthus fragrans (Thunb.) Lour., and explore the suitable temperature condition for pollen storage of sweet Osmanthus. Taking the pol-lens of nine sweet osmanthus cultivars of four groups as test material, the pollens were preserved in room temperature, low temperature, freezing and supper-low temperature. The pollen viabilities during different storage time were deter-mined. The results indicated that the pollen germination was well developed in the solid medium composed by sucrose 10%, H3 BO3 0?01% and agarose 1%. The germination rate could be calculated after 24 hours cultivating under a con-stant temperature of 25 ℃. The light and dark showed no significant effect on pollen germination. The ability of pollen germination demonstrateed a significant difference in different storage conditions. Under room temperature ( 15-20℃) , O. fragrans pollen could survive after 15-20 days. In low temperature, pollen life can be extended to 50 days. In freezing temperature and supper-low temperature, pollen life can be extended longer, and the most( 78%) pollen germination of cultivated O. fragrans showed no significant difference after 100 days. Pollen vitality of O. fragrans decline slower under the condition of freezing temperature and supper-low temperature, it can be preserved for longer.
