CAJ | 학술논문

目的：通过建立快速高效的UFLC-MS/MS方法同时检测分析胆汁酸类化合物，以便于更好地定量分析胆汁酸的在体水平．方法：以甘草次酸为内标，采用超高效液相与质谱联用技术同时测定13个胆汁酸类化合物，并评价该方法的精密度，准确度，及线性范围．结果：建立了能同时检测以甘草次酸为内标13个胆汁酸化合物的UFLC/MS /MS 检测方法，各成分分离度好，13种胆汁酸的浓度在1～20000 nmol/L 的浓度范围有良好的线性关系；r 在0．995～0．999之间；检出限为0．065～9．37 nmol/L；方法的日内和日间准确度在97％～105％之间，日间日内精密度均小于8％．结论：建立的UFLC/MS /MS 方法灵敏度高，特异性好，适用于胆汁酸体外的分析研究．
목적：통과건립쾌속고효적UFLC-MS/MS방법동시검측분석담즙산류화합물，이편우경호지정량분석담즙산적재체수평．방법：이감초차산위내표，채용초고효액상여질보련용기술동시측정13개담즙산류화합물，병평개해방법적정밀도，준학도，급선성범위．결과：건립료능동시검측이감초차산위내표13개담즙산화합물적UFLC/MS /MS 검측방법，각성분분리도호，13충담즙산적농도재1～20000 nmol/L 적농도범위유량호적선성관계；r 재0．995～0．999지간；검출한위0．065～9．37 nmol/L；방법적일내화일간준학도재97％～105％지간，일간일내정밀도균소우8％．결론：건립적UFLC/MS /MS 방법령민도고，특이성호，괄용우담즙산체외적분석연구．
AIM:To quantitative analyze the level of bile acids in vivo,a rapid and efficient UFLC-MS/MS analysis method was performed to detect multiple bile acids.METHODS:Compound glycyrrhetinic acid was used as the internal standard (IS).Ultra-fast liquid chromatography-mass spectrometry technology was ap-plied to simultaneously detect 13 bile acids compounds.Also the accuracy,precision and the linear range of the method was as-sessed.RESULTS:An UFLC/MS/MS analysis method which could respectively detect 13 bile acids and IS glycyrrhetinic was established.All 13 bile acids compounds were obtained well line-ar relationship with concentration range of 1 ~20000 nmol/L.The limit of detection (LOD)were defined 0.065 ~9.37 nmol/L. The intra-and inter-day accuracy ranged from 97%~105%,and the intra- and inter-day precision were all less than 8%.CON-CLUSION:The UFLC/MS/MS method with good sensitivity and selectivity could be applied to investigate bile acids in vitro.