CAJ | 학술논문

目的：探讨CD82对着床窗口期小鼠子宫内膜上皮细胞内整合素αV、β3、E-cadherin以及β-catenin蛋白表达的影响。方法将构建的CD82腺病毒转染原代培养的小鼠子宫内膜上皮细胞。检测妊娠小鼠子宫内膜上皮细胞转染CD82腺病毒后，细胞内整合素αV、β3、E-cadherin和β-catenin的表达变化情况。结果提取的上皮细胞纯度为（93.2±0.6）％。构建的CD82腺病毒转染效率达到（92.0±4.5）％，转染原代培养的小鼠子宫内膜上皮细胞24 h后，RT-PCR检测发现CD82基因表达明显升高。转染48 h后，Western blot检测CD82蛋白水平明显升高。免疫细胞化学检测妊娠第4天的小鼠子宫内膜上皮细胞转染CD82腺病毒后，整合素αV、β3以及β-catenin的表达较未转染组均有明显上升（P＜0.05），但E-cadherin的表达量无明显变化（P＞0.05）。结论胚胎植入前， CD82可能影响小鼠子宫内膜上皮细胞内整合素αV、β3和β-catenin的蛋白表达。
목적：탐토CD82대착상창구기소서자궁내막상피세포내정합소αV、β3、E-cadherin이급β-catenin단백표체적영향。방법장구건적CD82선병독전염원대배양적소서자궁내막상피세포。검측임신소서자궁내막상피세포전염CD82선병독후，세포내정합소αV、β3、E-cadherin화β-catenin적표체변화정황。결과제취적상피세포순도위（93.2±0.6）％。구건적CD82선병독전염효솔체도（92.0±4.5）％，전염원대배양적소서자궁내막상피세포24 h후，RT-PCR검측발현CD82기인표체명현승고。전염48 h후，Western blot검측CD82단백수평명현승고。면역세포화학검측임신제4천적소서자궁내막상피세포전염CD82선병독후，정합소αV、β3이급β-catenin적표체교미전염조균유명현상승（P＜0.05），단E-cadherin적표체량무명현변화（P＞0.05）。결론배태식입전， CD82가능영향소서자궁내막상피세포내정합소αV、β3화β-catenin적단백표체。
Objective Uterine epithelial cells were isolated from pregnant mice and cultured in vitro , and exam-ined the effect of CD82 on the expression of integrin αV,β3, E-cadherin and β-catenin in the cells.Methods The uter-ine epithelial cells were primarily isolated from pregnant mouse uterus .The recombinant adenovirus containing mouse CD 82 gene which had been constructed in our lab infected the uterine epithelial cells .Immunocytochemistry was used to detect the protein expressions of integrin αV,β3, E-cadherin and β-catenin in the uterine epithelial cells , which were infected with CD82 adenovirus or not .Results 1.The purity of primary cultured cells was (93.2 ±0.6)%.2.The transfection efficiency of CD82 recombinant adenovirus was ( 92 ±4.5 )%.The adenoviral particles carrying CD 82 gene indeed ex-pressed CD82 gene and protein in the primary uterine epithelial cells after 24 hours or 48 hours.3.The uterine epithelial cells of pregnant mice on d4 expressed integrin αV, β3, E-cadherin and β-catenin.4.In contrast to the control group, when CD82 adenovirus infected cells , the uterine epithelial cells of pregnant mice on d 4 increased the expression of integrinαV,β3 and β-catenin protein, had no significant changes of E-cadherin.Conclusions CD82 may have effect on the ex-pression of integrin αV,β3 and β-catenin in mouse uterine epithelial cells before implantation .