CAJ | 학술논문

目的：从亚细胞超微结构的角度揭示其抗冻能力优于正常孵化胚胎的原因。方法利用透射电子显微镜观察小鼠休眠胚胎与正常孵化期胚胎在细胞连接和各细胞器形态与分布上的差异，以及冻融培养后的变化，并进行相关比较分析。结果通过亚细胞结构对比分析发现：冷冻前小鼠休眠胚胎为紧缩状，处于能量代谢较低的“基态”，通过冻融后培养，细胞器结构恢复与正常孵化胚胎冷冻前相似；而正常孵化胚胎经过冻融后，线粒体数量减少，细胞核松散，异染色质增多。结论小鼠休眠胚胎与正常孵化胚胎冻融后相比，其细胞状态更有利于物质储存及能量代谢，表明小鼠休眠胚胎从亚细胞超微结构的角度比其正常孵化胚胎更具抗冻性。
목적：종아세포초미결구적각도게시기항동능력우우정상부화배태적원인。방법이용투사전자현미경관찰소서휴면배태여정상부화기배태재세포련접화각세포기형태여분포상적차이，이급동융배양후적변화，병진행상관비교분석。결과통과아세포결구대비분석발현：냉동전소서휴면배태위긴축상，처우능량대사교저적“기태”，통과동융후배양，세포기결구회복여정상부화배태냉동전상사；이정상부화배태경과동융후，선립체수량감소，세포핵송산，이염색질증다。결론소서휴면배태여정상부화배태동융후상비，기세포상태경유리우물질저존급능량대사，표명소서휴면배태종아세포초미결구적각도비기정상부화배태경구항동성。
Objective The aim of this study was to investigate the differences of the cell ultrastucture of normal mouse hatched blastocysts and their dormant ones cultured in vitro after freezing-thawing, and to explore whether the dor-mant embryos have a better anti-freezing shock property than the normal hatched mouse embryos .Methods By transmis-sion electron microscopy , the ultrastructure of these two types of mouse embryos was observed and analyzed .Results By comparative analysis of their ultrastructure , the results showed that the dormant embryos before freezing are being austerity and with lower energy metabolism at a ‘ground state ’ .After freezing-thawing and culture , their cellular structure seemed to be similar to that of the normal embryos cultured in vitro before freezing.However, after freezing-thawing and culture, the number of mitochondria decreased , the nuclei were loose , and their heterochromatin also increased .Conclusions From the ultrastructural observation , compared with the normal mouse hatched embryos , the cellular state of dormant mouse em-bryos after freezing-thawing is more favorable for material storage and energy metabolism , thus, indicating that they have a better anti-freezing property than normal hatched embryos .