CAJ | 학술논문

目的：应用胆总管内注射无水乙醇建立SD大鼠胆道闭锁模型。方法将SD雄性大鼠随机分为实验组和对照组，在实验组中经静脉留置针插入胆总管注入无水乙醇，对照组注入生理盐水。观察SD大鼠的生化及病理结果。结果在实验组中SD大鼠根据病理及生化检测分为肝功能持续恶化组和肝功能修复组，肝功能持续恶化组在8周以后生化明显高于对照组及肝功能修复组。常规HE染色及SMA、Masson染色也出现明显变化。结论胆总管无水乙醇注射诱导胆道闭锁模型是一种可靠的动物模型，此动物模型会帮助人们进一步研究胆道闭锁提供更多的研究手段。
목적：응용담총관내주사무수을순건립SD대서담도폐쇄모형。방법장SD웅성대서수궤분위실험조화대조조，재실험조중경정맥류치침삽입담총관주입무수을순，대조조주입생리염수。관찰SD대서적생화급병리결과。결과재실험조중SD대서근거병리급생화검측분위간공능지속악화조화간공능수복조，간공능지속악화조재8주이후생화명현고우대조조급간공능수복조。상규HE염색급SMA、Masson염색야출현명현변화。결론담총관무수을순주사유도담도폐쇄모형시일충가고적동물모형，차동물모형회방조인문진일보연구담도폐쇄제공경다적연구수단。
Objective To establish a new rat model of biliary atresia by pure ethanol injection into the common bile duct.Methods A catheter was inserted and fixed in the common bile duct in male SD rats .Saline (8 rats) or pure ethanol (16 rats) was injected through the catheter ,respectively, and the biochemical and pathological changes in the rats were examined .Results SD rats in the experimental group were divided into a persistent injury and a restoration of liver dysfunction groups according to pathological and biochemical detection .In the persistent injury group , biochemical impair-ments were significantly higher at 8 weeks after ethanol injection than those in the control group and restoration group .Dis-tinct pathological changes in the liver were observed using HE , SMA, and Masson staining .Conclusions It is a reliable animal model of biliary atresia induced by injection of pure ethanol into the common bile duct in the rat .It will provide a useful tool in future studies of biliary atresia .