CAJ | 학술논문

目的：通过观察血管源性头痛清醒动物模型中Fos阳性细胞在三叉神经节及三叉神经脊束核尾侧亚核的分布情况，明确两种非甾体类抗炎药NSAID对乙酰氨基酚及布洛芬在头痛控制中，在颅内特定区域的作用机理。方法30只雄性SD大鼠随机分为对照组（生理盐水组）、对乙酰氨基酚组、布洛芬组，每组给药后50 min分别给予频率为20 Hz、电流为3～5 mA和脉宽为0.25 ms的电刺激，刺激后给予大鼠灌注固定取脑，分别在颅内取三叉神经节及三叉神经脊束核尾侧亚核制作石蜡切片，进行免疫组织化学染色，利用Image J软件对阳性细胞进行计数统计。结果电刺激后盐水组与非甾体类药物组在双侧三叉神经节、三叉神经脊束核尾侧亚核Fos蛋白表达的差异具有显著统计学意义，对乙酰氨基酚组与布洛芬组在双侧三叉神经节、三叉神经脊束核尾侧亚核Fos蛋白表达未见统计学差异。结论给予非甾体类抗炎前后在双侧三叉神经节、三叉神经脊束核尾侧亚核的Fos表达的改变提示三叉神经节、三叉神经脊束核尾侧亚核参与了疼痛的传递和表达以及药物对疼痛控制的药理过程。
목적：통과관찰혈관원성두통청성동물모형중Fos양성세포재삼차신경절급삼차신경척속핵미측아핵적분포정황，명학량충비치체류항염약NSAID대을선안기분급포락분재두통공제중，재로내특정구역적작용궤리。방법30지웅성SD대서수궤분위대조조（생리염수조）、대을선안기분조、포락분조，매조급약후50 min분별급여빈솔위20 Hz、전류위3～5 mA화맥관위0.25 ms적전자격，자격후급여대서관주고정취뇌，분별재로내취삼차신경절급삼차신경척속핵미측아핵제작석사절편，진행면역조직화학염색，이용Image J연건대양성세포진행계수통계。결과전자격후염수조여비치체류약물조재쌍측삼차신경절、삼차신경척속핵미측아핵Fos단백표체적차이구유현저통계학의의，대을선안기분조여포락분조재쌍측삼차신경절、삼차신경척속핵미측아핵Fos단백표체미견통계학차이。결론급여비치체류항염전후재쌍측삼차신경절、삼차신경척속핵미측아핵적Fos표체적개변제시삼차신경절、삼차신경척속핵미측아핵삼여료동통적전체화표체이급약물대동통공제적약리과정。
Objective To define the functional mechanisms of two NSAIDs , paracetamol and ibuprofen , in specif-ic brain regions in headache control by observing the distribution of Fos -immunoreactive neurons in trigeminal ganglia and trigeminal nucleus caudalis in conscious rat models of vasculogenic headache .Methods Thirty male Sprague-Dawley rats were randomly divided into three groups: control group ( saline group ) , acetaminophen group and ibuprofen group .Each rat was given electrical stimulation ( frequency 20 Hz, current 3-5 mA, pulse width 0.25 ms) at 50 minutes after injec-tion .The rats were killed and perfusion fixed after electrical stimulation .Trigeminal ganglia and trigeminal nucleus caudalis of the brains were taken out for paraffin sections and immunohistochemical staining , and Fos-immunoreactive neurons were counted under the Image J system .Results After electrical stimulation , there were significant differences of Fos protein expression in bilateral trigeminal ganglia and spinal trigeminal nucleus caudalis between the saline group and groups of non -steroidal anti-inflammatory drugs , but no significant difference of Fos protein expression in bilateral trigeminal ganglia and spinal trigeminal nucleus caudalis between the acetaminophen group and ibuprofen group .Conclusions The changes of Fos expression in bilateral trigeminal ganglia and spinal trigeminal nucleus caudalis after treatment with NSAIDs suggest that such structures participate in the pain transmission and expression and the pharmacology course of analgesic drugs .