中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年
3期
30-34
,共5页
汪珊珊%汪电雷%陶秀华%汪辰吟%陈金佩%杨丽丽%曹银
汪珊珊%汪電雷%陶秀華%汪辰吟%陳金珮%楊麗麗%曹銀
왕산산%왕전뢰%도수화%왕신음%진금패%양려려%조은
脂多糖%慢性阻塞性肺疾病%多药耐药相关蛋白1%功能%表达%大鼠
脂多糖%慢性阻塞性肺疾病%多藥耐藥相關蛋白1%功能%錶達%大鼠
지다당%만성조새성폐질병%다약내약상관단백1%공능%표체%대서
Lipopolysaccharide%Chronic obstructive pulmonary disease%Multidrug resistance-associated protein 1%Function%Expression%Rat
目的：分析脂多糖（ LPS）造模方法对慢性阻塞性肺疾病（ COPD）模型大鼠的肺支气管上皮细胞多药耐药相关蛋白1（MRP1）功能的影响。方法利用LPS造模方法制备COPD模型大鼠，设置正常对照组、造模14 d组和造模28 d组，分别测定其呼吸功能；以酚红外排水平评价大鼠肺支气管上皮MRP1的功能；同时采用免疫组化法分析各组大鼠肺支气管上皮MRP1的表达。结果与正常对照组比较，LPS处理组造模进程中随时间的延长大鼠的各项肺功能指标明显下降；静脉给予酚红后其BALF中酚红浓度与血浆酚红浓度的比值降低；其肺支气管上皮MRP1蛋白表达显著性降低。结论 LPS造模方法制备COPD模型大鼠，随着造模的进程，其肺支气管上皮细胞MRP1蛋白的功能随之下调。
目的：分析脂多糖（ LPS）造模方法對慢性阻塞性肺疾病（ COPD）模型大鼠的肺支氣管上皮細胞多藥耐藥相關蛋白1（MRP1）功能的影響。方法利用LPS造模方法製備COPD模型大鼠，設置正常對照組、造模14 d組和造模28 d組，分彆測定其呼吸功能；以酚紅外排水平評價大鼠肺支氣管上皮MRP1的功能；同時採用免疫組化法分析各組大鼠肺支氣管上皮MRP1的錶達。結果與正常對照組比較，LPS處理組造模進程中隨時間的延長大鼠的各項肺功能指標明顯下降；靜脈給予酚紅後其BALF中酚紅濃度與血漿酚紅濃度的比值降低；其肺支氣管上皮MRP1蛋白錶達顯著性降低。結論 LPS造模方法製備COPD模型大鼠，隨著造模的進程，其肺支氣管上皮細胞MRP1蛋白的功能隨之下調。
목적：분석지다당（ LPS）조모방법대만성조새성폐질병（ COPD）모형대서적폐지기관상피세포다약내약상관단백1（MRP1）공능적영향。방법이용LPS조모방법제비COPD모형대서，설치정상대조조、조모14 d조화조모28 d조，분별측정기호흡공능；이분홍외배수평평개대서폐지기관상피MRP1적공능；동시채용면역조화법분석각조대서폐지기관상피MRP1적표체。결과여정상대조조비교，LPS처리조조모진정중수시간적연장대서적각항폐공능지표명현하강；정맥급여분홍후기BALF중분홍농도여혈장분홍농도적비치강저；기폐지기관상피MRP1단백표체현저성강저。결론 LPS조모방법제비COPD모형대서，수착조모적진정，기폐지기관상피세포MRP1단백적공능수지하조。
Objective To study the impact of establishment of lipopolysaccharide ( LPS )-induced rat model of chronic obstructive pulmonary disease (COPD)on the function of multidrug resistance-associated protein 1(MRP1)in the rat bronchial epithelium .Methods Using intratracheal instillation of LPS to establish COPD rat model .8-week old healthy male Wistar rats were divided into 3 groups (10 rats in each group ):(1) Normal control;(2) Modeling for 14 days after LPS instillation;(3) Modeling for 28 days after LPS instillation.Pulmonary function and the concentration of phenol red in bronchoalveolar lavage fluid ( BALF) and plasma were measured .The ratio of phenol red concentration in BALF/plasma was used as an index of the MRP 1 function in the rat bronchial epithelium and the expression of MRP 1 in the bronchial epi-thelium was also observed by immunohistochemistry .Results Compared with the normal group , the pulmonary functions of the rats in the model groups were significantly reduced along with the modeling progress .After intravenous administration of phenol red, the ratio of phenol red concentration in BALF/plasma was gradually reduced , and the expression of MRP1 in the bronchial epithelium was significantly decreased .Conclusions COPD rat model can be established by intratracheal LPS instillation, and the function of MRP1 in bronchial epithelium was gradually reduced along with the modeling progress .
