CAJ | 학술논문

目的：探讨cAMP／PKA-pCREB信号通路是否在康复训练促进的缺血性脑卒中大鼠运动功能的恢复方面发挥作用。方法采用Longa改良线栓法制备大鼠大脑中动脉缺血再灌注模型（ middle cerebral artery ische-mia-reperfusion model，MCAO），造模成功的大鼠随机分为自然恢复组（n ＝24）、自然恢复＋Rp-cAMP组（n ＝24）、康复训练组（n ＝18）和康复训练＋Rp-cAMP组（n ＝18）。同时设立假手术组（n ＝12）。于侧脑室注射Rp-cAMP后立即进行MCAO模型的制备。训练组大鼠于术后48 h开始每天给予平衡木、转棒及滚筒训练。采用平衡木试验评定大鼠的运动功能。酶联免疫法（ELISA）检测缺血灶周围的脑组织内PKA表达，蛋白免疫印迹法（West-ern blot）检测pCREB蛋白表达，同时采用免疫组化法对pCREB进行定位检测。结果（1）运动功能评分结果揭示，自然恢复＋Rp-cAMP组大鼠的运动功能低于自然恢复组，提示Rp-cAMP可抑制脑缺血大鼠运动神经功能的恢复；康复训练组大鼠的运动功能明显高于自然恢复组，也高于康复训练＋Rp-cAMP组，提示Rp-cAMP明显减弱康复训练促进脑缺血大鼠运动神经功能的恢复；（2）于术后2 d、7 d、14 d、21 d检测缺血灶周围的脑组织PKA、pCREB的蛋白表达结果显示：康复训练组明显高于自然恢复组，同时高于康复训练＋Rp-cAMP组，提示康复训练促进脑缺血大鼠的PKA、pCREB蛋白的表达，且Rp-cAMP明显抑制了康复训练促进脑缺血大鼠的PKA、pCREB蛋白的表达。结论 cAMP／PKA-pCREB信号通路可能介导康复训练促进的脑缺血大鼠运动功能的恢复。
목적：탐토cAMP／PKA-pCREB신호통로시부재강복훈련촉진적결혈성뇌졸중대서운동공능적회복방면발휘작용。방법채용Longa개량선전법제비대서대뇌중동맥결혈재관주모형（ middle cerebral artery ische-mia-reperfusion model，MCAO），조모성공적대서수궤분위자연회복조（n ＝24）、자연회복＋Rp-cAMP조（n ＝24）、강복훈련조（n ＝18）화강복훈련＋Rp-cAMP조（n ＝18）。동시설립가수술조（n ＝12）。우측뇌실주사Rp-cAMP후립즉진행MCAO모형적제비。훈련조대서우술후48 h개시매천급여평형목、전봉급곤통훈련。채용평형목시험평정대서적운동공능。매련면역법（ELISA）검측결혈조주위적뇌조직내PKA표체，단백면역인적법（West-ern blot）검측pCREB단백표체，동시채용면역조화법대pCREB진행정위검측。결과（1）운동공능평분결과게시，자연회복＋Rp-cAMP조대서적운동공능저우자연회복조，제시Rp-cAMP가억제뇌결혈대서운동신경공능적회복；강복훈련조대서적운동공능명현고우자연회복조，야고우강복훈련＋Rp-cAMP조，제시Rp-cAMP명현감약강복훈련촉진뇌결혈대서운동신경공능적회복；（2）우술후2 d、7 d、14 d、21 d검측결혈조주위적뇌조직PKA、pCREB적단백표체결과현시：강복훈련조명현고우자연회복조，동시고우강복훈련＋Rp-cAMP조，제시강복훈련촉진뇌결혈대서적PKA、pCREB단백적표체，차Rp-cAMP명현억제료강복훈련촉진뇌결혈대서적PKA、pCREB단백적표체。결론 cAMP／PKA-pCREB신호통로가능개도강복훈련촉진적뇌결혈대서운동공능적회복。
Objective To explore whether the cAMP-PKA-pCREB signal pathway plays a role in promoting the recovery of motor function after rehabilitation training in cerebral ischemia-reperfusion rats .Methods The middle cerebral artery occlusion model ( MCAO) was established by modified Longa nylon occlusion method in adult male Sprague -Dawley rats.The 84 MCAO rats were selected and randomly assigned to four groups:the natural recovery group without any special training (group B, n=24),natural recovery group with Rp-cAMP (group C, n=24), rehabilitation training group (group D, n=18) and rehabilitation training with Rp-cAMP (group E, n=18), and in addition a control group (group A, n =12).To establish rat MCAO models immediately after injection of Rp-cAMP into the lateral ventricle of the brain .The rats in the groups D and E were trained by balance beam , bar rotating and rolling exercises started at 48 h after MCAO.The ex-pression of PKA was determined by enzyme-linked immunosorbent assay ( ELISA) and the pCREB protein expression was detected by Western blot assay .Motor function was assessed by balance beam test .Results (1) The motor function score in the group C was significantly higher than that of group B , suggesting that Rp-cAMP inhibited the recovery of motor func-tion in the cerebral ischemia-reperfusion rats .The score of group D was significantly lower than that of groups B and E , in-dicating that Rp-cAMP inhibited the promoting effect of rehabilitation training on motor function in the cerebral ischemia -reperfusion rats.(2) The expressions of PKA and pCREB proteins detected at 2nd, 7th, 14th, and 21th days after surgery showed that their expressions in the group D were significantly higher than those of the groups B and E , indicating that re-habilitation training promoted the expression of PKA and pCREB , and Rp-cAMP significantly inhibited the promoting effect of rehabilitation training on the expressions of PKA and pCREB proteins .Conclusion cAMP/PKA-pCREB signal trans-duction pathway may mediate a promoting effect of rehabilitation training on the recovery of motor function after ischemic stroke in rats.