中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年
3期
1-6
,共6页
孙世惠%吴小红%刘晨风%高同同%曾扬%郭彦%唐健%潘婷%于虹%寇志华%赵光宇%周育森
孫世惠%吳小紅%劉晨風%高同同%曾颺%郭彥%唐健%潘婷%于虹%寇誌華%趙光宇%週育森
손세혜%오소홍%류신풍%고동동%증양%곽언%당건%반정%우홍%구지화%조광우%주육삼
H7 N9病毒%H1 N1病毒%肺损伤%免疫反应%小鼠
H7 N9病毒%H1 N1病毒%肺損傷%免疫反應%小鼠
H7 N9병독%H1 N1병독%폐손상%면역반응%소서
H7N9 Virus%H1N1 Virus%Lung Injury%Pathology%Immune Response%Mice
目的：比较分析H7 N9病毒与H1 N1病毒感染小鼠病理学损伤特点，初步探讨两种病毒感染致小鼠急性肺损伤的致病机制。方法 H7 N9病毒与H1 N1病毒分别感染小鼠，观察不同病毒感染后小鼠生存率，并于不同时间点取心、肝、脾、肺、肾、脑、肠等组织，伊红-苏木素染色并进行组织病理学分析，免疫组化检测病毒抗原分布及中性粒细胞浸润。综合分析肺组织病理损伤与病毒复制、宿主免疫反应之间的关系。结果 H7 N9病毒感染小鼠肺及脾脏损伤较轻，存活率较高。 H1 N1病毒感染的小鼠肺及脾脏损伤较重，感染后9 d全部死亡；两种病毒抗原主要分布于支气管上皮细胞、少量间质细胞和肺泡上皮细胞，病毒复制水平无明显差异。但H1 N1病毒感染后肺及脾脏中均有大量中性粒细胞浸润，小鼠机体炎症反应明显强于H7 N9病毒感染后小鼠炎症反应。结论 H7 N9病毒与H1 N1病毒感染后小鼠病理学损伤特点及程度均不同，病毒复制是小鼠肺损伤的诱发因素但并非决定因素，宿主针对病毒感染产生的免疫反应程度与急性肺损伤密切相关。
目的：比較分析H7 N9病毒與H1 N1病毒感染小鼠病理學損傷特點，初步探討兩種病毒感染緻小鼠急性肺損傷的緻病機製。方法 H7 N9病毒與H1 N1病毒分彆感染小鼠，觀察不同病毒感染後小鼠生存率，併于不同時間點取心、肝、脾、肺、腎、腦、腸等組織，伊紅-囌木素染色併進行組織病理學分析，免疫組化檢測病毒抗原分佈及中性粒細胞浸潤。綜閤分析肺組織病理損傷與病毒複製、宿主免疫反應之間的關繫。結果 H7 N9病毒感染小鼠肺及脾髒損傷較輕，存活率較高。 H1 N1病毒感染的小鼠肺及脾髒損傷較重，感染後9 d全部死亡；兩種病毒抗原主要分佈于支氣管上皮細胞、少量間質細胞和肺泡上皮細胞，病毒複製水平無明顯差異。但H1 N1病毒感染後肺及脾髒中均有大量中性粒細胞浸潤，小鼠機體炎癥反應明顯彊于H7 N9病毒感染後小鼠炎癥反應。結論 H7 N9病毒與H1 N1病毒感染後小鼠病理學損傷特點及程度均不同，病毒複製是小鼠肺損傷的誘髮因素但併非決定因素，宿主針對病毒感染產生的免疫反應程度與急性肺損傷密切相關。
목적：비교분석H7 N9병독여H1 N1병독감염소서병이학손상특점，초보탐토량충병독감염치소서급성폐손상적치병궤제。방법 H7 N9병독여H1 N1병독분별감염소서，관찰불동병독감염후소서생존솔，병우불동시간점취심、간、비、폐、신、뇌、장등조직，이홍-소목소염색병진행조직병이학분석，면역조화검측병독항원분포급중성립세포침윤。종합분석폐조직병리손상여병독복제、숙주면역반응지간적관계。결과 H7 N9병독감염소서폐급비장손상교경，존활솔교고。 H1 N1병독감염적소서폐급비장손상교중，감염후9 d전부사망；량충병독항원주요분포우지기관상피세포、소량간질세포화폐포상피세포，병독복제수평무명현차이。단H1 N1병독감염후폐급비장중균유대량중성립세포침윤，소서궤체염증반응명현강우H7 N9병독감염후소서염증반응。결론 H7 N9병독여H1 N1병독감염후소서병이학손상특점급정도균불동，병독복제시소서폐손상적유발인소단병비결정인소，숙주침대병독감염산생적면역반응정도여급성폐손상밀절상관。
Objective To analyze and compare the pathological changes of lung tissue in mice infected with the novel H7N9 influenza virus and 2009 pandemic H1N1 influenza virus, respectively, and to preliminarily study the mecha-nisms of acute lung injury induced by those virus infection .Methods SPF 6-week old BALB/c mice ( body weight 18-20 g, male∶female=1∶1) (n=3 in each subgroup) were intranasally infected with H7N9 virus and H1N1 virus, respec-tively.The behavior and survival time of mice after virus infection were observed and the survival rates were analyzed .The heart, liver, spleen, lung, kidney, intestines, and brain were collected at indicated time points for histopathological exami-nation using H&E staining .The distribution of virus antigen was detected by immunohistochemistry .The neutrophil infiltra-tion was also observed .The correlation of lung injury with virus replication and host immune responses was analyzed .Re-sults The lung and spleen injury of mice infected with H 7N9 virus was slighter and their survival rate (100%) was high-er than those of mice infected with H1N1 virus.The damages of the lung and spleen in H1N1virus-infected mice were more severe than that in H7N9 virus-infected mice, and all the 10 mice in this group died within 9 days after virus inoculation . The distributions of both the virus antigens were mainly in the bronchial epithelial cells , a few stromal cells and alveolar ep-ithelial cells .The levels of virus replication in the two groups were not significantly different .There were more intense neu-trophil infiltration in the lung and inflammatory response in the H 1N1 virus-infected mice than those in the H7N9 virus-in-fected mice .Conclusions There are some differences of the pathological characteristics and extent of lung injury in the mice infected with H7N9 virus and H1N1 virus, respectively.The virus replication is a precipitating factor but not the deci-sive factor of the lung injury , and there is a close relationship between the host immune responses and acute lung injury .
