中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
3期
169-171
,共3页
人参皂苷Rg1%二苯乙烯苷%正交实验%高效液相色谱法%超声提取%回流提取
人參皂苷Rg1%二苯乙烯苷%正交實驗%高效液相色譜法%超聲提取%迴流提取
인삼조감Rg1%이분을희감%정교실험%고효액상색보법%초성제취%회류제취
Ginsenosides Rg1%Stilbene glycoside%orthogonal experiment%high performance liquid chromatography%ultrasonic extraction%reflux extraction
目的:优选人参首乌胶囊回流制备工艺。比较回流提取、渗漉提取对人参首乌胶囊有效成分的影响。方法按正交实验设计,高效液相色谱法(high performance liquid chromatography,HPLC)测定人参皂苷Rg1、二苯乙烯苷含量,以人参皂苷Rg1和二苯乙烯苷综合转移率(11)为指标,分别考察了乙醇浓度(%)、乙醇用量(倍)、提取时间、提取次数(次)对回流提取的影响;比较了回流提取与渗漉提取的优劣。结果最优提取方法为8倍50%乙醇加热回流提取1次,每次1.5 h。结论该制备方法简便、可行、稳定性高,为工业生产提供依据。
目的:優選人參首烏膠囊迴流製備工藝。比較迴流提取、滲漉提取對人參首烏膠囊有效成分的影響。方法按正交實驗設計,高效液相色譜法(high performance liquid chromatography,HPLC)測定人參皂苷Rg1、二苯乙烯苷含量,以人參皂苷Rg1和二苯乙烯苷綜閤轉移率(11)為指標,分彆攷察瞭乙醇濃度(%)、乙醇用量(倍)、提取時間、提取次數(次)對迴流提取的影響;比較瞭迴流提取與滲漉提取的優劣。結果最優提取方法為8倍50%乙醇加熱迴流提取1次,每次1.5 h。結論該製備方法簡便、可行、穩定性高,為工業生產提供依據。
목적:우선인삼수오효낭회류제비공예。비교회류제취、삼록제취대인삼수오효낭유효성분적영향。방법안정교실험설계,고효액상색보법(high performance liquid chromatography,HPLC)측정인삼조감Rg1、이분을희감함량,이인삼조감Rg1화이분을희감종합전이솔(11)위지표,분별고찰료을순농도(%)、을순용량(배)、제취시간、제취차수(차)대회류제취적영향;비교료회류제취여삼록제취적우렬。결과최우제취방법위8배50%을순가열회류제취1차,매차1.5 h。결론해제비방법간편、가행、은정성고,위공업생산제공의거。
Objective To screen and optimize the preparation process of Radix Ginseng Capsules.This paper compares the reflux extraction and effects of infiltration percolation extraction to the renshenshouwu capsules.Methods Based on the Orthogonal test,the content of ginsenosides Rg1 and Stilbene glycoside were measured by HPLC.The transferring rates of ginsenosides Rg1 and Stilbene glycoside (1:1 )were regarded as the index to evaluate extracting conditions ,such as the ethanol concentration (%),consumption of alcohol (times),extraction time and extraction times (times). Results The optimum extract conditions of ginsenosides Rg1 and Stilbene glycoside are:with 8 times volume of 50%concentration ethanol as the extract liquid,to refluxing extract 1 times,1.5 h every time.Conclusion The preparation method is simple,practical,high stability and provides the basis for industrial production.