CAJ | 학술논문

目的：探讨肺炎支原体肺炎（mycoplasma pneumonia，MPP）患儿T淋巴细胞亚群、D-二聚体和免疫球蛋白的变化及其临床意义。方法应用流式细胞仪检测技术测定肺炎支原体肺炎患儿（MPP组，n＝50）和健康儿童（正常对照组，n＝50）CD3＋、CD4＋、CD8＋、CD4＋/CD8＋的水平；免疫比浊法检测2组中D-二聚体水平；免疫透射比浊法检测2组的血清免疫球蛋白（IgA、IgM、IgG）水平。结果 T淋巴细胞亚群检测结果：与正常对照组比较，MPP组外周血CD3＋和CD4＋细胞降低差异显著（P＜0.001）、CD8＋细胞升高无显著差异、CD4＋/CD8＋比值降低无显著差异。D-二聚体水平检测结果：与正常对照组比较，MPP组D-二聚体水平升高差异显著（P＜0.001）。免疫球蛋白检测结果：MPP组血清IgA、IgM较正常对照组相比，差异显著（P＜0.05），MPP组血清IgG与正常对照组比较差异无统计学意义。结论肺炎支原体肺炎患儿体内免疫功能紊乱，受到不同程度抑制，凝血功能异常，故支原体肺炎患儿除了常规抗肺炎支原体治疗外，需根据不同的临床症状和疾病发展情况，监测D-二聚体水平的表达，并加强对免疫系统的调节和治疗。
목적：탐토폐염지원체폐염（mycoplasma pneumonia，MPP）환인T림파세포아군、D-이취체화면역구단백적변화급기림상의의。방법응용류식세포의검측기술측정폐염지원체폐염환인（MPP조，n＝50）화건강인동（정상대조조，n＝50）CD3＋、CD4＋、CD8＋、CD4＋/CD8＋적수평；면역비탁법검측2조중D-이취체수평；면역투사비탁법검측2조적혈청면역구단백（IgA、IgM、IgG）수평。결과 T림파세포아군검측결과：여정상대조조비교，MPP조외주혈CD3＋화CD4＋세포강저차이현저（P＜0.001）、CD8＋세포승고무현저차이、CD4＋/CD8＋비치강저무현저차이。D-이취체수평검측결과：여정상대조조비교，MPP조D-이취체수평승고차이현저（P＜0.001）。면역구단백검측결과：MPP조혈청IgA、IgM교정상대조조상비，차이현저（P＜0.05），MPP조혈청IgG여정상대조조비교차이무통계학의의。결론폐염지원체폐염환인체내면역공능문란，수도불동정도억제，응혈공능이상，고지원체폐염환인제료상규항폐염지원체치료외，수근거불동적림상증상화질병발전정황，감측D-이취체수평적표체，병가강대면역계통적조절화치료。
Objective To explore the changes of T-lymphocyte subsets,D-Dimer and immunoglobulin in mycoplasma pneumonia children and its significance.Methods The levels of T lymphocyte subsets (CD3 +、CD4 +、CD8 +、CD4 +/CD8 +)in 50 children with mycoplasma pneumonia (MPP Group)and 50 healthy children were detected by flow cytometry;The levels of D-Dimer were detected by immunoturbidimetry;The levels of serum immunoglobulin were detected by immune turbidimetric method.Results The results of T lymphocyte subsets:Compared with normal control group, CD3 +and CD4 +peripheral blood cells in MPP group were significantly declined(P<0.001)、CD8 +cells were increased slightly,but with no significant difference、CD4 +/CD8 +ratios were decreased slightly.The results of D-Dimer:Compared with normal control group,the levels of D-Dimer in MPP group were significantly escalated(P<0.001).The results of immunoglobulin:IgA and IgMin MPP group were both significantly higher than those of normal group,but IgG was not different from that in normal group.Conclusion Children with mycoplasma pneumonia have immune disorder,which may generate abnormal clotting.Therefore,besides the conventional anti-mycoplasma pneumonia treatment,we need to detect the levels of D-Dimer according to the clinical symptoms and disease progression and strength the regulation and treatment of the immune system.