CAJ | 학술논문

目的：探讨Clara细胞分泌蛋白（Clara cell secretion protein 16，CC16）对创伤性休克大鼠模型继发性肺损伤的保护作用及其机制。方法选择30只成年雄性SD大鼠，随机均分为3组：假手术组、对照组、CC16处理组（n＝10）。采用创伤性休克动物模型，假手术组均完成基本手术操作，但不放血和复苏；对照组放血后在液体复苏前雾化吸入与CC16处理组等容量的生理盐水；CC16处理组在复苏前给予雾化吸入0.1μg/mL的重组人Clara细胞分泌蛋白（reconstituted Clara cell secretion protein，rh-CCSP）。实验结束后监测动脉血气分析及肺湿/干重比等指标变化，并测定肺组织中丙二醛（malondialdehyde，MDA）、髓过氧化物酶（myeloperoxidase， MPO）及肺组织病理学的改变。结果与对照组相比，CC16处理组的pH、PaO2值显著升高，BE值显著降低（P＜0.05），肺湿/干重比显著减少（P＜0.05）；CC16处理组与假手术组间各项指标比较无统计学意义；与对照组相比，CC16处理组的MDA、MP0含量明显降低（P＜0.05），肺组织病理学形态也有改善（P＜0.05）。结论雾化吸入rh-CCSP可减轻创伤性休克大鼠继发性肺损伤的炎症反应，对肺组织具有保护作用，机制可能与其抗氧化应用有关。
목적：탐토Clara세포분비단백（Clara cell secretion protein 16，CC16）대창상성휴극대서모형계발성폐손상적보호작용급기궤제。방법선택30지성년웅성SD대서，수궤균분위3조：가수술조、대조조、CC16처리조（n＝10）。채용창상성휴극동물모형，가수술조균완성기본수술조작，단불방혈화복소；대조조방혈후재액체복소전무화흡입여CC16처리조등용량적생리염수；CC16처리조재복소전급여무화흡입0.1μg/mL적중조인Clara세포분비단백（reconstituted Clara cell secretion protein，rh-CCSP）。실험결속후감측동맥혈기분석급폐습/간중비등지표변화，병측정폐조직중병이철（malondialdehyde，MDA）、수과양화물매（myeloperoxidase， MPO）급폐조직병이학적개변。결과여대조조상비，CC16처리조적pH、PaO2치현저승고，BE치현저강저（P＜0.05），폐습/간중비현저감소（P＜0.05）；CC16처리조여가수술조간각항지표비교무통계학의의；여대조조상비，CC16처리조적MDA、MP0함량명현강저（P＜0.05），폐조직병이학형태야유개선（P＜0.05）。결론무화흡입rh-CCSP가감경창상성휴극대서계발성폐손상적염증반응，대폐조직구유보호작용，궤제가능여기항양화응용유관。
Objective To investigate the effect and mechanism of Clara cell secretory protein (CC16 ) on lung injury of traumatic shock rats.Methods Thirty SD male rats were randomly assigned to three groups:sham-operation group,control group and CC16-treatment group.The rat model of traumatic shock was used in this study.Rats of sham-operation group were operated,but not treated by depletion and recovery.Rats of control group inhaled the physiological saline before fluid resuscitation.Rats of CC16-treatment group inhaled 0.1 μg/mL of recombinant human Clara cell secretory protein (rh-CCSP)before fluid resuscitation.Arterial blood gas analysis and wet/dry weight were detected in each group.The contents of malondialdehyde (MDA),myeloperoxidase (MPO)of lung tissues and lung tissue pathology changes were also studied. Results Compared with control group,pH and PaO2 value in CC16-treatment group increased significantly.Meanwhile,BE value and lung wet/dry weight ratio also decreased significantly (P<0.05).Compared with control group,contents of MDA and MPO in CC16-treatment group decreased significantly (P<0.05).Lung tissue pathology improved in the CC16 group compared with that of control group (P <0.05 ).Conclusion Inhaled Rh-CCSP may have potential protective effect on lung injury tissues of traumatic shock rats model through its antioxidative effect.