CAJ | 학술논문

目的：研究异硫氰酸苯乙酯（phenethyl isothiocyanate，PEITC）抑制肺癌细胞NCI-H446生长的机制。方法体外培养肺癌NCI-H446细胞，并分为对照组和PEITC处理组。对照组细胞给予0．1％二甲基亚砜处理，PEITC组分别用10μmol/L、30μmol/L和50μmol/L PEITC作用24 h，噻唑蓝法和流式细胞术分别检测细胞的增殖与凋亡；Western blot检测Akt磷酸化、细胞内活性caspase-3和caspase-9的表达以及胞浆中细胞色素C的含量；Real time PCR检测磷酸酯酶与张力蛋白同源物（phosphatase and tensin homologue， PTEN）mRNA水平。结果 PEITC可显著抑制肺癌NCI-H446细胞增殖（P＜0.05），并诱导其凋亡。PEITC处理后，肺癌细胞内活性caspase-3和caspase-9含量显著高于对照组（P＜0.05）；同时，PEITC 处理后，肺癌细胞质中细胞色素C 含量明显增高（P＜0.05）。PEITC也能抑制NCI-H446细胞中Akt的磷酸化水平。此外，PEITC处理后，可明显诱导肺癌细胞内PTEN表达的增加（P＜0.05），其中50μmol/L PEITC处理后，PTEN mRNA含量增高了4.6倍。结论 PEITC能抑制NCI-H446肺癌细胞生长，其机制可能通过上调PETN表达，从而抑制PI3K/Akt的活性有关。
목적：연구이류청산분을지（phenethyl isothiocyanate，PEITC）억제폐암세포NCI-H446생장적궤제。방법체외배양폐암NCI-H446세포，병분위대조조화PEITC처리조。대조조세포급여0．1％이갑기아풍처리，PEITC조분별용10μmol/L、30μmol/L화50μmol/L PEITC작용24 h，새서람법화류식세포술분별검측세포적증식여조망；Western blot검측Akt린산화、세포내활성caspase-3화caspase-9적표체이급포장중세포색소C적함량；Real time PCR검측린산지매여장력단백동원물（phosphatase and tensin homologue， PTEN）mRNA수평。결과 PEITC가현저억제폐암NCI-H446세포증식（P＜0.05），병유도기조망。PEITC처리후，폐암세포내활성caspase-3화caspase-9함량현저고우대조조（P＜0.05）；동시，PEITC 처리후，폐암세포질중세포색소C 함량명현증고（P＜0.05）。PEITC야능억제NCI-H446세포중Akt적린산화수평。차외，PEITC처리후，가명현유도폐암세포내PTEN표체적증가（P＜0.05），기중50μmol/L PEITC처리후，PTEN mRNA함량증고료4.6배。결론 PEITC능억제NCI-H446폐암세포생장，기궤제가능통과상조PETN표체，종이억제PI3K/Akt적활성유관。
Objective To investigate the anti-proliferation mechanisms of Phenethyl isothiocyanate (PEITC)in human lung cancer cells line NCI-H446. Methods Human lung cancer cell line NCI-H446 was cultured in vitro,and treated with 10,30,50μmol/L PEITC for 24 h respectively. Cell viability and apoptosis were analyzed by methylthiazolyldiphenyl-tetrazolium bromide (MTT)assay and flow cytometry,respectively. Phosphorylation of Akt,activation of caspase-3 and caspase-9 in NCI-H446 cells,and production of cytochrome C in cytoplasm were detected by Western blot. Expression of phosphatase and tensin homologue (PTEN)was detected by real-time PCR. Results PEITC significantly reduced NCI-H446 cells proliferation in dose-dependent manner (P<0.05 ),and apoptosis was also inducted after PEITC administration. PEITC also markedly induced expression of caspase-3 and caspase-9,as compared with control group. And the level of cytochrome C in the cytoplasm was also increased after treatment with PEITC.Furthermore,PEITC treatment significantly increased the mRNA level of PTEN in NCI-H446 cells. Conclusion PEITC may be used as a potential anti-lung cancer agent by regulationg PI3K/AKT pathway and increasing PETN expression.