中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2014年
3期
31-33,36
,共4页
内皮祖细胞%肺动脉高压%TXA2%PGI2
內皮祖細胞%肺動脈高壓%TXA2%PGI2
내피조세포%폐동맥고압%TXA2%PGI2
endothelial progenitor cells%pulmonary arterial hypertension%TXA2%PGI2
目的：探讨移植内皮祖细胞（endothelial progenitor cells，EPCs）后，野百合碱（monocrotaline，MCT）诱导兔肺动脉高压（pulmonary artery hypertension，PHA）模型中血浆血栓素A2（thromboxane A2，TXA2）、前列腺素I2（prostaglandin I2，PGI2）水平的变化。方法建立野百合碱诱导兔肺动脉高压模型；体外分离诱导兔内皮祖细胞，并进行鉴定；将50只新西兰大白兔随机分为3组，正常对照组和模型组经尾静脉注射M199培养基，EPCs治疗组经尾静脉注射荧光标记的EPCs。3周后测定兔血浆血栓素B2（thromboxane B2，TXB2）和6-酮-前列腺素1α（6-keto-prostagland in F1 alpha，6-keto-PGF1α）的含量，并计算2者的比值。结果与模型组相比，EPCs治疗组血浆中6-keto-PGFlα的含量显著增加，为（130.67±17.54）μmol/L；TXB2的含量显著降低，为（402.89±27.98）μmol/L；2组TXB2/6-keto-PGF1α的比值差异有统计学意义（P＜0.01）。结论移植内皮祖细胞可以降低由野百合碱诱导的肺动脉高压中TXA2的含量及增加PGI2的含量，从而逆转肺动脉高压的损伤。
目的：探討移植內皮祖細胞（endothelial progenitor cells，EPCs）後，野百閤堿（monocrotaline，MCT）誘導兔肺動脈高壓（pulmonary artery hypertension，PHA）模型中血漿血栓素A2（thromboxane A2，TXA2）、前列腺素I2（prostaglandin I2，PGI2）水平的變化。方法建立野百閤堿誘導兔肺動脈高壓模型；體外分離誘導兔內皮祖細胞，併進行鑒定；將50隻新西蘭大白兔隨機分為3組，正常對照組和模型組經尾靜脈註射M199培養基，EPCs治療組經尾靜脈註射熒光標記的EPCs。3週後測定兔血漿血栓素B2（thromboxane B2，TXB2）和6-酮-前列腺素1α（6-keto-prostagland in F1 alpha，6-keto-PGF1α）的含量，併計算2者的比值。結果與模型組相比，EPCs治療組血漿中6-keto-PGFlα的含量顯著增加，為（130.67±17.54）μmol/L；TXB2的含量顯著降低，為（402.89±27.98）μmol/L；2組TXB2/6-keto-PGF1α的比值差異有統計學意義（P＜0.01）。結論移植內皮祖細胞可以降低由野百閤堿誘導的肺動脈高壓中TXA2的含量及增加PGI2的含量，從而逆轉肺動脈高壓的損傷。
목적：탐토이식내피조세포（endothelial progenitor cells，EPCs）후，야백합감（monocrotaline，MCT）유도토폐동맥고압（pulmonary artery hypertension，PHA）모형중혈장혈전소A2（thromboxane A2，TXA2）、전렬선소I2（prostaglandin I2，PGI2）수평적변화。방법건립야백합감유도토폐동맥고압모형；체외분리유도토내피조세포，병진행감정；장50지신서란대백토수궤분위3조，정상대조조화모형조경미정맥주사M199배양기，EPCs치료조경미정맥주사형광표기적EPCs。3주후측정토혈장혈전소B2（thromboxane B2，TXB2）화6-동-전렬선소1α（6-keto-prostagland in F1 alpha，6-keto-PGF1α）적함량，병계산2자적비치。결과여모형조상비，EPCs치료조혈장중6-keto-PGFlα적함량현저증가，위（130.67±17.54）μmol/L；TXB2적함량현저강저，위（402.89±27.98）μmol/L；2조TXB2/6-keto-PGF1α적비치차이유통계학의의（P＜0.01）。결론이식내피조세포가이강저유야백합감유도적폐동맥고압중TXA2적함량급증가PGI2적함량，종이역전폐동맥고압적손상。
Objective To discuss the changes of plasma TXA2 and PGI2 levels in the rabbit model of pulmonary hypertension (PHA)induced by monocrotaline (MCT)when transplanted with endothelial progenitor cells (EPCs). Methods The rabbit pulmonary hypertension model was constructed by MCT induction. Rabbit endothelial progenitor cells were induced,separated and identified in vitro.50 New Zealand white rabbits were randomly divided into 3 groups,normal control group and model group were given M199 culture medium by tail vein injection,EPCs treatment group were given EPCs marked with fluorescent though tail vein injection. The levels of plasma TXB2 and 6-keto-PGF1αwere detected and their ratio were calculated after three weeks. Results Compared with model group,the content of 6-keto-PGFlαin plasma in EPCs group was (130.67 ±17.54)u mol/L,which was increased sinnificantly. The content of TXB2 was (402.89 ±27.98 )u mol/L,which was decreased significantly.The ratio of TXB2 to 6-keto-PGF1 alpha between two groups was statistically significant(P<0.01 ). Conclusion EPCs transplantation can reduce the content of TXA2 and increase the content of PGI2 in the pulmonary hypertension rabbits induced by monocrotaline,then reverse the damage of pulmonary hypertension.
