CAJ | 학술논문

目的：研究重症胰腺炎患者p38 MAPK基因和蛋白的表达量，探讨p38 MAPK在重症胰腺炎炎症反应的作用。方法选取2012年1月～2013年12月牡丹江红旗医院收治的48例重症胰腺炎患者作为实验组，同期在本院进行体检的48例健康人为对照组。采用荧光实时定量PCR检测2组受试者p38 MAPK的mRNA表达；Western Blot检测p38 MAPK蛋白及P-p38 MAPK蛋白的表达。结果 RT-PCR结果显示实验组重症胰腺炎患者的p38 MAPK基因的表达量明显高于对照组48例健康体检者的p38 MAPK基因表达量，差异有统计学意义（P＜0.05）；Western结果表明实验组重症胰腺炎患者的p38 MAPK和P-p38 MAPK蛋白表达量均明显高于对照组，差异有统计学意义（P＜0.05）。结论重症胰腺炎患者p38 MAPK基因和蛋白表达量均明显升高，表明p38 MAPK在重症胰腺炎炎症反应可能扮演重要角色。
목적：연구중증이선염환자p38 MAPK기인화단백적표체량，탐토p38 MAPK재중증이선염염증반응적작용。방법선취2012년1월～2013년12월모단강홍기의원수치적48례중증이선염환자작위실험조，동기재본원진행체검적48례건강인위대조조。채용형광실시정량PCR검측2조수시자p38 MAPK적mRNA표체；Western Blot검측p38 MAPK단백급P-p38 MAPK단백적표체。결과 RT-PCR결과현시실험조중증이선염환자적p38 MAPK기인적표체량명현고우대조조48례건강체검자적p38 MAPK기인표체량，차이유통계학의의（P＜0.05）；Western결과표명실험조중증이선염환자적p38 MAPK화P-p38 MAPK단백표체량균명현고우대조조，차이유통계학의의（P＜0.05）。결론중증이선염환자p38 MAPK기인화단백표체량균명현승고，표명p38 MAPK재중증이선염염증반응가능분연중요각색。
Objective To investigate the expression of p38 MAPK gene and p38 MAPK protein,and explore the effect of p38 MAPK in inflammation reaction of severe acute pancreatitis.Methods 48 cases with severe acute pancreatitis were chosen as research group,and another 48 healthy cases as control group.The expression of p38 MAPK gene were investigated by RT-PCR,and the expression of p38 MAPK and P-p38 MAPK protein were investigated by western blot. Results The expression of p38 MAPK mRNA,p38 MAPK protein and P-p38 MAPK protein in research group were higher than that in control group,the differences were all statistically significant(P<0.05 ).Conclusion The expression of p38 MAPK gene and p38 MAPK protein were significantly increased in patients with severe acute pancreatitis,which showed that p38 MAPK played an important role in inflammation reaction.