CAJ | 학술논문

目的：探讨5-氮杂-2'-脱氧胞苷（5-Aza-2'-deoxycytidine，5-Aza-CdR）对胰腺癌细胞株增殖的影响以及对其原钙黏附素（protocadherin 8，PCDH8）基因表达的影响。方法用不同浓度的5-Aza-CdR处理胰腺癌Capan-2细胞。阴性对照组不加药，根据5-Aza-CdR处理的剂量分为：0.08μmol/L组、0.40μmol/L组、2.00μmol/L组、10μmol/L组、50μmol/L组，检测细胞的生长情况；采用RT-PCR和Western Blot检测各组胰腺癌细胞PCDH8基因和蛋白的表达。结果5-Aza-CdR对胰腺癌细胞具有明显的抑制作用，且随着浓度的增加，抑制率逐渐增强（P＜0.01）；但是随着时间的增加，抑制率逐渐下降（P＜0.01）。5-Aza-CdR和吉西他滨联用对胰腺癌细胞的抑制作用和诱导凋亡作用最强（P＜0.05，P＜0.01）。5-Aza-CdR能显著增加胰腺癌细胞PCDH8基因和PCDH8蛋白的表达水平，且随着浓度的升高，增加作用越明显（P＜0.01）。结论5-Aza-CdR能够抑制胰腺癌细胞的增殖，增加PCDH8基因的表达，与吉西他滨联合后抑制作用更强。
목적：탐토5-담잡-2'-탈양포감（5-Aza-2'-deoxycytidine，5-Aza-CdR）대이선암세포주증식적영향이급대기원개점부소（protocadherin 8，PCDH8）기인표체적영향。방법용불동농도적5-Aza-CdR처리이선암Capan-2세포。음성대조조불가약，근거5-Aza-CdR처리적제량분위：0.08μmol/L조、0.40μmol/L조、2.00μmol/L조、10μmol/L조、50μmol/L조，검측세포적생장정황；채용RT-PCR화Western Blot검측각조이선암세포PCDH8기인화단백적표체。결과5-Aza-CdR대이선암세포구유명현적억제작용，차수착농도적증가，억제솔축점증강（P＜0.01）；단시수착시간적증가，억제솔축점하강（P＜0.01）。5-Aza-CdR화길서타빈련용대이선암세포적억제작용화유도조망작용최강（P＜0.05，P＜0.01）。5-Aza-CdR능현저증가이선암세포PCDH8기인화PCDH8단백적표체수평，차수착농도적승고，증가작용월명현（P＜0.01）。결론5-Aza-CdR능구억제이선암세포적증식，증가PCDH8기인적표체，여길서타빈연합후억제작용경강。
Objective To discuss the effect of 5-Aza-CdR on pancreatic carcinoma Capan-2 cell proliferation and PCDH8 gene expression.Methods Pancreatic carcinoma Capan-2 cells were treated with different doses of 5-Aza-CdR with or without gemcitabine,negative control group without drug,0.08μmol/L group with 0.08μmol/L 5-Aza-CdR,0.40 μmol/L group with 0.40 μmol/L 5-Aza-CdR,2.00 μmol/L group with 2.00 μmol/L 5-Aza-CdR,10 μmol/L group with 10 μmol/L 5-Aza-CdR,50 μmol/L group with 50 μmol/L 5-Aza-CdR. The inhibition ratio of Capan-2 cell proliferation were observed by MTT assay and PCDH8 gene and protein expression were detected by RT-PCR and Western blot. Results The inhibition ratio was increased with 5-Aza-CdR dose increasing(P<0.01),but decreased apparently with times extending(P<0.01). Inhibition ratio in 5-Aza-CdR and gemcitabine group was higher than those with only 5-Aza-CdR or gemcitabine groups(P<0.05 or P<0.01).The levels of PCDH8 gene and protein expression were increased significantly in 5-Aza-CdR treatment groups,with dose dependent (P <0.01 ).Conclusion 5-Aza-CdR can inhibit the proliferation of pancreatic carcinoma Capan-2 cell proliferation,and increase PCDH8 gene expression. The inhibition effect is strong when combined with gemcitabine.