CAJ | 학술논문

目的：观察补中益气汤含药血清对肺腺癌耐顺铂细胞株（A549/DDP）磷脂酰肌醇3激酶（phosphatidylinositol 3 kinase， PI3K）、丝/苏氨酸蛋白激酶（protein kinase B，AKT）、生存素（Survivin）蛋白表达的影响，探讨其作用机制。方法制备中剂量补中益气汤含药血清（0.576 g/mL），将A549/DDP细胞分成4组：空白血清组、最佳含药血清组、LY294002组、最佳含药血清组＋LY294002组（即联合组）。采用免疫细胞化学和免疫蛋白印迹法（western blot）方法检测PI3K、AKT和Survivin蛋白的表达。结果最佳含药血清组和联合组PI3K、AKT、Survivin蛋白的表达显著低于空白血清组（P＜0.05），联合组PI3K、AKT、Survivin蛋白减少的更加明显，但与最佳含药血清组相比无统计学差异；LY294002组Survivin蛋白的表达明显低于空白血清对照组，差异有统计学意义（P＜0.05）。结论补中益气汤含药血清作用于PI3K/AKT信号转导通路，能降低肺腺癌顺铂耐药细胞株PI3K、AKT、Survivin蛋白的表达，提高A549/DDP细胞对顺铂的敏感性。
목적：관찰보중익기탕함약혈청대폐선암내순박세포주（A549/DDP）린지선기순3격매（phosphatidylinositol 3 kinase， PI3K）、사/소안산단백격매（protein kinase B，AKT）、생존소（Survivin）단백표체적영향，탐토기작용궤제。방법제비중제량보중익기탕함약혈청（0.576 g/mL），장A549/DDP세포분성4조：공백혈청조、최가함약혈청조、LY294002조、최가함약혈청조＋LY294002조（즉연합조）。채용면역세포화학화면역단백인적법（western blot）방법검측PI3K、AKT화Survivin단백적표체。결과최가함약혈청조화연합조PI3K、AKT、Survivin단백적표체현저저우공백혈청조（P＜0.05），연합조PI3K、AKT、Survivin단백감소적경가명현，단여최가함약혈청조상비무통계학차이；LY294002조Survivin단백적표체명현저우공백혈청대조조，차이유통계학의의（P＜0.05）。결론보중익기탕함약혈청작용우PI3K/AKT신호전도통로，능강저폐선암순박내약세포주PI3K、AKT、Survivin단백적표체，제고A549/DDP세포대순박적민감성。
Objective To observe the effect of Buzhong Yiqi Decoction medicated serum on PI3K、AKT、survivin in lung adenocarcinoma A549/DDP cells,and explore its functional mechanism.Methods Buzhong Yiqi Decoction medicated serum of middle dosage (0.576 g/mL)were prepared,and A549/DDP cells were divided into four groups:blank serum group,the best containing medicine serum group and LY294002 group (blockers group), the best containing medicine serum +LY294002 group (combination group).The protein expression of PI3K,AKT and Survivin were detected by Immunocytochemistry and Western blot.Results Compare with blank serum group,the protein expression of PI3K、AKT and Survivin in best containing medicine serum and combination group were significantly lower(P<0.05 ),and the later was more lower,but there was no significant differences between best containing medicine serum and combination group.The expression of survivin in LY294002 group were significantly lower than blank serum group(P<0.05 ).Conclusion Buzhong Yiqi Decoction can reduce the protein expression of PI3K,AKT and surviving,increase the sensitivity of A549/DDP to DDP through PI3K/AKT signal transduction pathway.