包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2014年
3期
9-12,23
,共5页
王素华%孙景萍%高艳荣%张营
王素華%孫景萍%高豔榮%張營
왕소화%손경평%고염영%장영
姜黄素%氧化损伤%纳米二氧化硅%超氧化物歧化酶%丙二醛%总抗氧化能力%谷胱甘肽过氧化物酶
薑黃素%氧化損傷%納米二氧化硅%超氧化物歧化酶%丙二醛%總抗氧化能力%穀胱甘肽過氧化物酶
강황소%양화손상%납미이양화규%초양화물기화매%병이철%총항양화능력%곡광감태과양화물매
Curcumin%Oxidative Damage%SOD%MDA%GSH-PX%T-AOC%Nm-sized silicon dioxide
目的：研究姜黄素对纳米级二氧化硅（nm - SiO2）粉尘诱导的大鼠肺损伤的抗氧化作用。方法：Wistar 雄性大鼠50只，随机分为阴性对照组、模型组及姜黄素干预高、中、低剂量组，每组10只。气管内1次性灌注纳米级二氧化硅悬液（15 mg/100 g）复制大鼠肺损伤模型，姜黄素分别按200、100、50 mg/ kg·d 于大鼠造模后第2 d 灌胃，4周后取动物血、肝、肺组织测定氧化损伤，另取一侧肺组织制作病理检查。结果：模型组大鼠体重低于阴性对照组（ P ﹤0.05），肺脏系数高于阴性对照组（ P ﹤0.01）。姜黄素各剂量组大鼠体重均高于模型组（ P ﹤0.05）；与阴性对照组比较，模型组血清、肺组织匀浆、肝组织匀浆中超氧化物歧化酶（superoxide dismutase，SOD）活性、谷胱甘肽过氧化物酶（glutathione per-oxidase，GSH - Px）活性和总抗氧化能力（total - antioxide capacity，T - AOC）明显降低（ P ﹤0.01），丙二醛（malondialde-hyde，MDA）含量增高（ P ﹤0.05）；与模型组比较，血清、肺组织匀浆、肝组织匀浆中 MDA 含量降低；各剂量组两两比较，肺组织匀浆中 MDA 含量差异有统计学意义（ P ﹤0.05）；随着姜黄素剂量的增加，呈一定的剂量反应关系。其中血清、肺组织匀浆、肝组织匀浆中 SOD 活性、GSH - Px 活性及 T - AOC 含量呈现增高趋势，MDA 含量呈现下降趋势。结论：姜黄素能对抗大鼠氧化损伤的过程，缓解由纳米二氧化硅粉尘引起的氧化损伤。
目的：研究薑黃素對納米級二氧化硅（nm - SiO2）粉塵誘導的大鼠肺損傷的抗氧化作用。方法：Wistar 雄性大鼠50隻，隨機分為陰性對照組、模型組及薑黃素榦預高、中、低劑量組，每組10隻。氣管內1次性灌註納米級二氧化硅懸液（15 mg/100 g）複製大鼠肺損傷模型，薑黃素分彆按200、100、50 mg/ kg·d 于大鼠造模後第2 d 灌胃，4週後取動物血、肝、肺組織測定氧化損傷，另取一側肺組織製作病理檢查。結果：模型組大鼠體重低于陰性對照組（ P ﹤0.05），肺髒繫數高于陰性對照組（ P ﹤0.01）。薑黃素各劑量組大鼠體重均高于模型組（ P ﹤0.05）；與陰性對照組比較，模型組血清、肺組織勻漿、肝組織勻漿中超氧化物歧化酶（superoxide dismutase，SOD）活性、穀胱甘肽過氧化物酶（glutathione per-oxidase，GSH - Px）活性和總抗氧化能力（total - antioxide capacity，T - AOC）明顯降低（ P ﹤0.01），丙二醛（malondialde-hyde，MDA）含量增高（ P ﹤0.05）；與模型組比較，血清、肺組織勻漿、肝組織勻漿中 MDA 含量降低；各劑量組兩兩比較，肺組織勻漿中 MDA 含量差異有統計學意義（ P ﹤0.05）；隨著薑黃素劑量的增加，呈一定的劑量反應關繫。其中血清、肺組織勻漿、肝組織勻漿中 SOD 活性、GSH - Px 活性及 T - AOC 含量呈現增高趨勢，MDA 含量呈現下降趨勢。結論：薑黃素能對抗大鼠氧化損傷的過程，緩解由納米二氧化硅粉塵引起的氧化損傷。
목적：연구강황소대납미급이양화규（nm - SiO2）분진유도적대서폐손상적항양화작용。방법：Wistar 웅성대서50지，수궤분위음성대조조、모형조급강황소간예고、중、저제량조，매조10지。기관내1차성관주납미급이양화규현액（15 mg/100 g）복제대서폐손상모형，강황소분별안200、100、50 mg/ kg·d 우대서조모후제2 d 관위，4주후취동물혈、간、폐조직측정양화손상，령취일측폐조직제작병리검사。결과：모형조대서체중저우음성대조조（ P ﹤0.05），폐장계수고우음성대조조（ P ﹤0.01）。강황소각제량조대서체중균고우모형조（ P ﹤0.05）；여음성대조조비교，모형조혈청、폐조직균장、간조직균장중초양화물기화매（superoxide dismutase，SOD）활성、곡광감태과양화물매（glutathione per-oxidase，GSH - Px）활성화총항양화능력（total - antioxide capacity，T - AOC）명현강저（ P ﹤0.01），병이철（malondialde-hyde，MDA）함량증고（ P ﹤0.05）；여모형조비교，혈청、폐조직균장、간조직균장중 MDA 함량강저；각제량조량량비교，폐조직균장중 MDA 함량차이유통계학의의（ P ﹤0.05）；수착강황소제량적증가，정일정적제량반응관계。기중혈청、폐조직균장、간조직균장중 SOD 활성、GSH - Px 활성급 T - AOC 함량정현증고추세，MDA 함량정현하강추세。결론：강황소능대항대서양화손상적과정，완해유납미이양화규분진인기적양화손상。
Objective:To explore the antioxidative effect of curcumin on the injured lung of the rats induced by nm - sized silicon dioxide(SiO2 )dust. Methods:50 male Wistar rats were randomized into 5 groups,namely,negative contrast group, model group and three curcumin intervention groups(high dosage,medium dosage and low dosage),with 10 rats in each group. Rats in both the model group and the three curcumin intervention groups were once given gastro - intillation of nm - sized SiO2 suspension(15 mg/ 100 g)through trachea to establish rat lung injury models. The rats from three curcumin intervention groups were given gastro - intillation of curcumin,with doses of 200,100 and 50 mg/ kg·d,two days after the establishment of the models. The tissues of hearts,blood,lung and the liver of the rats were taken to examine oxidative injury,with the tissues of the other side of the lung taken for pathological section. Results:The body weight of the rats in the model group was significantly lower than that in the negative contrast group(P ﹤ 0. 05),with the co - efficient of heart and lung higher than that in the nega-tive contrast group(P ﹤ 0. 01). The body weight of the rats in the three curcumin intervention groups was higher than that in the model group(P ﹤ 0. 05). Compared with that of those in the negative contrast group,the activity of superoxide dismutase (SOD),glutathione peroxidase(GSH - Px)and total - antioxide capacity(T - AOC)in serum,lung,and liver homogenate of the rats from the model group decreased significantly(P ﹤ 0. 01),with the content of malondialdehyde(MDA)significantly in-creased(P ﹤ 0. 05). Compared with that in the model group,MDA content decreased in the serum,lung,and the liver homog-enate of the rats from the three curcumin intervention groups;there was significant difference in MDA content among the three curcumin intervention groups(P ﹤ 0. 05),indicating a dosage - effect causal relation with increase in the dose of curcumin;the activity of SOD,GSH - Px and T - AOC increased in the serum,lung,and liver homogenate. Conclusion:Curcumin can fight off the process of oxidative damage in rats and alleviate the oxidative damage caused by nm - sized SiO2 .
