包头医学院学报
包頭醫學院學報
포두의학원학보
JOURNAL OF BAOTOU MEDICAL COLLEGE
2014年
3期
1-3
,共3页
王铭婕%安亮%丁海麦%李晓晶%杜晓红%闫朝丽%苏燕
王銘婕%安亮%丁海麥%李曉晶%杜曉紅%閆朝麗%囌燕
왕명첩%안량%정해맥%리효정%두효홍%염조려%소연
聚丙烯酰胺凝胶电泳%考马斯亮蓝 R250%考马斯亮蓝 G250%染色
聚丙烯酰胺凝膠電泳%攷馬斯亮藍 R250%攷馬斯亮藍 G250%染色
취병희선알응효전영%고마사량람 R250%고마사량람 G250%염색
Polyacrylamide gel electrophoresis%Coomassie brilliant blue R250%Coomassie brilliant blue G250%Staining
目的：比较考马斯亮蓝（Coomassie brilliant blue，CBB）R250和 G250两种凝胶染色方法的特点及染色效果。方法：用 R250和 G250两种染液在同一条件下对完全相同的两块聚丙烯酰胺凝胶进行染色，观察比较其染色及漂洗的时间、效果、灵敏度、安全性等指标。结果：CBB R250染色至少需要2 h，漂洗4 h 以上可见蛋白条带；CBB G250染色2 h 后不需漂洗即可见蛋白条带，漂洗2 h 后背景几乎无色，条带清晰。结论：G250染色在所需时间、效果、灵敏度、安全性和相关度等方面均优于 R250，适用于要求快速出结果的重复性好、稳定性高的染色。
目的：比較攷馬斯亮藍（Coomassie brilliant blue，CBB）R250和 G250兩種凝膠染色方法的特點及染色效果。方法：用 R250和 G250兩種染液在同一條件下對完全相同的兩塊聚丙烯酰胺凝膠進行染色，觀察比較其染色及漂洗的時間、效果、靈敏度、安全性等指標。結果：CBB R250染色至少需要2 h，漂洗4 h 以上可見蛋白條帶；CBB G250染色2 h 後不需漂洗即可見蛋白條帶，漂洗2 h 後揹景幾乎無色，條帶清晰。結論：G250染色在所需時間、效果、靈敏度、安全性和相關度等方麵均優于 R250，適用于要求快速齣結果的重複性好、穩定性高的染色。
목적：비교고마사량람（Coomassie brilliant blue，CBB）R250화 G250량충응효염색방법적특점급염색효과。방법：용 R250화 G250량충염액재동일조건하대완전상동적량괴취병희선알응효진행염색，관찰비교기염색급표세적시간、효과、령민도、안전성등지표。결과：CBB R250염색지소수요2 h，표세4 h 이상가견단백조대；CBB G250염색2 h 후불수표세즉가견단백조대，표세2 h 후배경궤호무색，조대청석。결론：G250염색재소수시간、효과、령민도、안전성화상관도등방면균우우 R250，괄용우요구쾌속출결과적중복성호、은정성고적염색。
Objective:To compare the characteristics and effects of coomassie brilliant blue(CCB)R250 and G250 stai-ning methods. Methods:Two identical polyacrylamide gel were stained by CBB G250 and R250 at the same condition,respec-tively. The time of staining and rinsing,effect,sensitivity and safety were compared. Results:It took at least 2 hours for staining and 4 hours for rinsing to show protein bands using CBB R250. However,the protein bands could be seen after 2 hour staining by CBB G250,and the bands were clear after 2 hour rinsing. Conclusion:The staining effct of CBB G250 is better than that of CBB R250 in sensitivity,dying and rinsing time,security etc. CBB G250 staining method can be applied to fast and stable staining with good reproducibility.
