CAJ | 학술논문

以胶体几丁质为唯一碳源，从大连渤海湾的底泥样品中分离到1株高产低温几丁质酶的海洋细菌，命名为DL-06。采用单因素优化方法确定该菌株产低温几丁质酶的最佳条件，结果表明：胶体几丁质5.0 g/L，胰蛋白胨5.0 g/L，发酵温度20℃，pH 7.0，2%接种量，装液量60%，转速130 r/min，陈海水1.0 L，发酵时间30 h，在该条件下酶活力达6.87 U/mL。
이효체궤정질위유일탄원，종대련발해만적저니양품중분리도1주고산저온궤정질매적해양세균，명명위DL-06。채용단인소우화방법학정해균주산저온궤정질매적최가조건，결과표명：효체궤정질5.0 g/L，이단백동5.0 g/L，발효온도20℃，pH 7.0，2%접충량，장액량60%，전속130 r/min，진해수1.0 L，발효시간30 h，재해조건하매활력체6.87 U/mL。
Using colloidal chitin as the sole carbon source, a marine bacteria with high productivity of cold-adapted chitinase was obtained from samples collected in Bohai bay in Dalian city of Liaoning Province, China,named as DL-06. The optimal fermentation conditions of cold-adapted chitinase-producing bacteria by single-factor experiments were determined as followed:colloidal chitin 5.0g/L, peptone 5.0 g/L, fermentation temperature 20℃, pH 7.0, inoculums dose 2%, shaking speed 130r/min, aged seawater 1.0 L and fermentation 30 h, respectively. Under these conditions, the maximum activity of cold-adapted chitinase was 6.87 U/ml.