CAJ | 학술논문

目的：研究医院感染的金黄色葡萄球菌的多重耐药机制，指导临床合理用药。方法对本院感染标本分离出的金黄色葡萄球菌进行药敏试验，聚合酶链反应（PCR）测定mecA、aac（6＇）/aph（2＇）、aph（3＇）-III、ermA 、ermC、tetM 和tetK基因。结果 MecA 基因检测阳性为84株，为 MRSA ，阳性率48％，其余91株为MSSA ；MRSA对12种抗菌药物敏感率均低于MSSA敏感率；MRSA与MSSA对耐药基因携带率各不相同，MRSA高于MSSA。结论医院感染的金黄色葡萄球菌对抗菌药物有很高的耐药性，金黄色葡萄球菌的多重耐药与获得mecA基因紧密相关。
목적：연구의원감염적금황색포도구균적다중내약궤제，지도림상합리용약。방법대본원감염표본분리출적금황색포도구균진행약민시험，취합매련반응（PCR）측정mecA、aac（6＇）/aph（2＇）、aph（3＇）-III、ermA 、ermC、tetM 화tetK기인。결과 MecA 기인검측양성위84주，위 MRSA ，양성솔48％，기여91주위MSSA ；MRSA대12충항균약물민감솔균저우MSSA민감솔；MRSA여MSSA대내약기인휴대솔각불상동，MRSA고우MSSA。결론의원감염적금황색포도구균대항균약물유흔고적내약성，금황색포도구균적다중내약여획득mecA기인긴밀상관。
Objective To study the multiple resistance mechanisms of hospital infection of staphylococcus aureus ,and to guide clinical rational drug use .Methods The susceptibility to antimicrobial agents were tested ,and mecA、aac(6ˊ)/aph(2")、aph (3ˊ)-III ,ermA、ermC ,tetM and tetK of 175 clinical Staphylococcus aureus were detected by polymerase chain reaction (PCR) methods .Results Among the 175 isolates of Staphylococci aureus ,84 isolates were MRSA(48% );MSSA and MRSA drug-re-sistant genes carry are not identical ,MSSA was higher than MRSA .Conclusion The hospital infection of staphylococcus aureus had high antimicrobial resistance .The multiple restance mechanism of staphylococcus aureus was closely associated with mecA gene .