CAJ | 학술논문

目的：建立有效的树鼩巴尔通体PCR检测方法，并进行初步应用。方法针对NCBI公布的巴尔通体序列设计3对引物，通过对我国主要流行菌株的扩增实验来确定1对引物，并进行体系优化、特异性和敏感性测试；运用该PCR方法对60份树鼩样品进行检测，阳性样本进行序列测定。结果所建树鼩巴尔通体PCR检测方法特异性良好，灵敏度较高（2.0×10-5μg／mL），60份树鼩样本中有15份阳性样本，通过序列测定证实树鼩巴尔通体感染率为25％，与扩增结果完全相符，验证了该方法的可行性。结论该方法的建立为树鼩巴尔通体的检测奠定了基础。
목적：건립유효적수구파이통체PCR검측방법，병진행초보응용。방법침대NCBI공포적파이통체서렬설계3대인물，통과대아국주요류행균주적확증실험래학정1대인물，병진행체계우화、특이성화민감성측시；운용해PCR방법대60빈수구양품진행검측，양성양본진행서렬측정。결과소건수구파이통체PCR검측방법특이성량호，령민도교고（2.0×10-5μg／mL），60빈수구양본중유15빈양성양본，통과서렬측정증실수구파이통체감염솔위25％，여확증결과완전상부，험증료해방법적가행성。결론해방법적건립위수구파이통체적검측전정료기출。
Objective To establish an effective PCR assay for detection of Bartonella, and application of this assay in tree shrew .Methods Sequence of Bartonella was obtained from NCBI Genbank .Three pairs of primers were designed based on this sequence .One pair of primers was determined through amplifying the major strains in China .Sixty tree shrew blood samples were tested with this PCR assay .The positive amplified fragments were sequenced to verify the reliability of this method .Results A PCR method for detection of Bartonella is successfully established , with a high specificity and the sensitivity was of 2.0 ×10 -5 μg/mL.Among the tested 60 blood samples , 15 positive cases were detected.Sequencing of the samples confirmed a 25%infection rate of Bartonella in the tree shrews, well consistent with the amplification results , and verified the applicability of this detection method .Conclusion The establishment of this method provides the basis for detection of Bartonella in tree shrew.