CAJ | 학술논문

目的：研究90 d摄入噻唑膦原药对大鼠的亚慢性毒性作用，确定其最大无作用剂量，为安全生产及慢性毒性实验提供剂量参考。方法将80只SD大鼠（雌雄各半）按体重随机分为4组，分别为噻唑膦原药0.8 mg／kg体重组、4.0 mg／kg体重组、20.0 mg／kg体重和正常对照组。在实验结束后处死实验大鼠，同时检测血清生化、体重、尿常规和脏器系数等指标，并对结果进行统计学处理。结果高剂量组雄、雌性大鼠体质量增长缓慢，雄性大鼠高剂量组甘油三酯（TG）、胆碱酯酶（CHE）明显低于对照组，雌性大鼠高剂量组碱性磷酸酶（ALP）明显高于对照组，雌性大鼠高剂量组总蛋白（ TP）、白蛋白（ ALB）、总胆红素（ TBIL）、肌酐（ CREA）、血糖（ GLU）、胆碱酯酶（CHE）明显低于对照组；雄、雌性大鼠尿胆红素（BIL）、比重（SG）、尿蛋白（PRO）的阳性率与对照组相比有显著性差异；雄性大鼠高剂量组脑、肺、肾、肾上腺、睾丸的脏器重量系数明显高于对照组，雌性大鼠高剂量组脑、肺、肾的脏器重量系数明显高于对照组，高剂量组的卵巢子宫的脏器重量系数明显低于对照组（ P＜0.05，P＜0.01）。结论噻唑膦原药对雄、雌性大鼠90经口染毒剂量为20.0 mg／kg 体重及以上时，对大鼠有毒性效应；90天喂养噻唑膦原药对大鼠最大无作用剂量为4 mg／kg 体重。
목적：연구90 d섭입새서련원약대대서적아만성독성작용，학정기최대무작용제량，위안전생산급만성독성실험제공제량삼고。방법장80지SD대서（자웅각반）안체중수궤분위4조，분별위새서련원약0.8 mg／kg체중조、4.0 mg／kg체중조、20.0 mg／kg체중화정상대조조。재실험결속후처사실험대서，동시검측혈청생화、체중、뇨상규화장기계수등지표，병대결과진행통계학처리。결과고제량조웅、자성대서체질량증장완만，웅성대서고제량조감유삼지（TG）、담감지매（CHE）명현저우대조조，자성대서고제량조감성린산매（ALP）명현고우대조조，자성대서고제량조총단백（ TP）、백단백（ ALB）、총담홍소（ TBIL）、기항（ CREA）、혈당（ GLU）、담감지매（CHE）명현저우대조조；웅、자성대서뇨담홍소（BIL）、비중（SG）、뇨단백（PRO）적양성솔여대조조상비유현저성차이；웅성대서고제량조뇌、폐、신、신상선、고환적장기중량계수명현고우대조조，자성대서고제량조뇌、폐、신적장기중량계수명현고우대조조，고제량조적란소자궁적장기중량계수명현저우대조조（ P＜0.05，P＜0.01）。결론새서련원약대웅、자성대서90경구염독제량위20.0 mg／kg 체중급이상시，대대서유독성효응；90천위양새서련원약대대서최대무작용제량위4 mg／kg 체중。
Objective To study the toxicity of fosthiazate feeding for 90 days in rats, and to determine the maximal non-effective dose of fosthiazate , in order to provide the reference dose for safety in production and chronic toxicity experiment.Methods A total of 80 SD rats ( half female and half male ) were randomly divided into 4 groups, respectively:0.8 mg/kg· bw· d group, 4.0 mg/kg· bw· d group, 20.0 mg/kg· bw· d group, and normal control group .The rats were sacrificed to determine the indices including serum biochemical parameters , body weight , routine urine test and organ coefficients after the end of the experiment , and the results were statistically analyzed .Results In the high dose group, the body weight gain was slowed in male and female rats .The TG and CHE in the high dose group of male rats and the TP, ALB, CREA, GLU, and CHE in the high dose group of female rats were significantly lower than those of normal control group.The ALP in the high dose group of female rats was higher than that of the normal control group .The positive rates of BIL, SG, and PRO in both male and female rats had significant differences compared with those of normal control group.The organ coefficients of brain , lung, kidney, adrenal, and testis of male rats, and the organ coefficients of brain , lung, and kidney of female rats in the high dose group were significantly higher than those of the normal control group .The ovaries and uterus in the female rats of high dose group were significantly lower than those of normal control group ( P<0.05,P<0.01).Conclusions The oral dose of fosthiazate at 4.0 mg/kg· bw· d fed for 90 days and above cause toxic effects on rats , and its maximal non-effect dose of long-term intake of low-dose fosthiazate on rats is 4 mg/kg· bw· d.