CAJ | 학술논문

目的：研究长爪沙鼠动情周期血清催乳素（PRL）、黄体生成素（LH）、促卵泡利激素（FSH）和雌二醇（ E2）水平和卵泡黄体数量的变化。方法采用直接镜检法确定长爪沙鼠的动情周期，以ELISA法测定长爪沙鼠血清PRL、E2、FSH 和LH水平，并观察动情周期各时相各级卵泡和黄体数量的变化情况。结果直接镜检法计数角化细胞统计阴道脱落细胞的类型及比例，确定长爪沙鼠动情周期的四个时相，发现长爪沙鼠动情周期各级卵泡数量各时相间均差异未见显著性。动情间期黄体数量较其他各时相显著减少（P＜0.05）。血清PRL和LH水平从动情前期至动情间期逐渐增高，至动情间期达峰值（ P＜0.05），而血清E2和FSH水平均在动情前期最高（ P＜0.05），在动情间期达到最低。结论长爪沙鼠动情周期各时相卵泡和黄体数量与各时相激素的变化密切相关，动情周期各时相卵泡数量差异未见显著性。动情周期中出现的血清FSH和E2峰值的时相与小大鼠一致，而PRL和LH出现峰值的时相与大小鼠有差异，为长爪沙鼠生殖生理特性的进一步研究提供了参考。
목적：연구장조사서동정주기혈청최유소（PRL）、황체생성소（LH）、촉란포리격소（FSH）화자이순（ E2）수평화란포황체수량적변화。방법채용직접경검법학정장조사서적동정주기，이ELISA법측정장조사서혈청PRL、E2、FSH 화LH수평，병관찰동정주기각시상각급란포화황체수량적변화정황。결과직접경검법계수각화세포통계음도탈락세포적류형급비례，학정장조사서동정주기적사개시상，발현장조사서동정주기각급란포수량각시상간균차이미견현저성。동정간기황체수량교기타각시상현저감소（P＜0.05）。혈청PRL화LH수평종동정전기지동정간기축점증고，지동정간기체봉치（ P＜0.05），이혈청E2화FSH수평균재동정전기최고（ P＜0.05），재동정간기체도최저。결론장조사서동정주기각시상란포화황체수량여각시상격소적변화밀절상관，동정주기각시상란포수량차이미견현저성。동정주기중출현적혈청FSH화E2봉치적시상여소대서일치，이PRL화LH출현봉치적시상여대소서유차이，위장조사서생식생리특성적진일보연구제공료삼고。
Objective To investigate the numbers of corpus luteum and ovarian follicles and compare the levels of serum prolactin (PRL), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2 ) in different phases of estrus cycle in female gerbils .Methods Consecutively taking vaginal smears of the gerbils and directly examined under light microscope to distinguish the four phases of the estrus cycle .Hematoxylin and eosin staining was used to histological examination of the gerbil ovaries , and to detect the levels of serum PRL , LH, FSH and E2 by ELISA assay during estrus cycle .Results The proportion of cornified vaginal exfolliated cells could be the basis to distinguish four phases respectively:proestrus, oestrus, metoestrus, and dioestrus.Moreover, there were no significant differences between the numbers of ovarian follicles in different phases of estrus cycle .The numbers of corpus luteum in preoestrus were significantly lower than that in the other phases of estrus cycle ( P <0.05 ) .The levels of serum PRL and LH were increasing constantly from preoestrus to dioestrus , and both reached a peak at dioestrus ( P<0.05 ) .The levels of serum FSH and E2 both peaked at preoestrus , and were significantly higher than those at oestrus , metoestrus and dioestrus ( P<0.05).Conclusions There are no significant differences between the numbers of ovarian follicles in different phases of estrus cycle .Gonadotropin , prolactin and estradiol paly important roles in the regulation of estrous cycle .The phases during which surges of FSH and E 2 occur in Mongolian gerbils are similar to those of rats and mice , while the PRL and LH are different .Our findings provide further reference to the study of reproductive physiology of Mongolian gerbils .