CAJ | 학술논문

目的：探讨嗜碱性粒细胞在胶原诱导性关节炎模型小鼠Th1／Th2失衡中的作用。方法取4～6周龄C57／BL6小鼠，经初次和3周后二次背部和足底多点胶原免疫，建立胶原诱导性关节炎小鼠模型。分别在建模（初次免疫）前、建模后1、3、6和9周眶静脉采血和采集淋巴结细胞，利用流式细胞术对嗜碱性粒细胞数量和表达IL-4的比例检测，ELISA方法检测血清IL-4和IFN-γ水平，并对模型进行评分。结果成功建立关节炎小鼠模型；模型建立后1周CIA模型小鼠血清IFN-γ／IL-4水平的比值显著低于建模前和对照组小鼠，提示以Th2应答为主，同时外周血中嗜碱性粒细胞的数量和表达IL-4的比例显著高于对照组；而建模后6和9周CIA模型小鼠血清IFN-γ／IL-4水平的比值显著高于对照组，提示以Th1应答为主，同时外周血中嗜碱性粒细胞的数量与对照组比较有降低的趋势。结论嗜碱性粒细胞可能通过影响Th1／Th2应答失衡参与CIA模型小鼠的发病。
목적：탐토기감성립세포재효원유도성관절염모형소서Th1／Th2실형중적작용。방법취4～6주령C57／BL6소서，경초차화3주후이차배부화족저다점효원면역，건립효원유도성관절염소서모형。분별재건모（초차면역）전、건모후1、3、6화9주광정맥채혈화채집림파결세포，이용류식세포술대기감성립세포수량화표체IL-4적비례검측，ELISA방법검측혈청IL-4화IFN-γ수평，병대모형진행평분。결과성공건립관절염소서모형；모형건립후1주CIA모형소서혈청IFN-γ／IL-4수평적비치현저저우건모전화대조조소서，제시이Th2응답위주，동시외주혈중기감성립세포적수량화표체IL-4적비례현저고우대조조；이건모후6화9주CIA모형소서혈청IFN-γ／IL-4수평적비치현저고우대조조，제시이Th1응답위주，동시외주혈중기감성립세포적수량여대조조비교유강저적추세。결론기감성립세포가능통과영향Th1／Th2응답실형삼여CIA모형소서적발병。
Objective To investigate the role of basophils in the imbalance of Th 1/Th2 response in mouse models of collagen-induced arthritis(CIA).Methods 4-6-weeks old C57/BL6 mice were immunized with collagen at multiple points on the back and foot twice (0 and 3 weeks) to establish a mouse model of collagen-induced arthritis.Blood samples were collected before the first immunization and 1, 3, 6 and 9 weeks after immunization , and cells from lymph nodes were collected.Flow cytometry and ELISA were employed to detect the levels of basophils and IL-4, and the joint swelling was scored.Results Mouse model of CIA was successful established .The ratio of IL-4/IFN-γof the CIA group was significantly lower than that in the mice before CIA modeling and the control group , indicating a Th2-dominant response .At the same time, the peripheral basophils counting and percentage of IL-4 positive basophils of the CIA group were significantly higher than those of the control group .While, the IL-4/IFN-γratio of the CIA group was significantly higher than that of the control group , indicating a Th1-dominant response .The peripheral basophils counting of the CIA group was slightly lower than that of the control group .Conclusion Basophils may participate in the development of CIA in mouse models through affecting the imbalance of Th 1/Th2 response.